FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004953554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20709
Sequences flagged as poor quality0
Sequence length35-300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN789238.10903471920421No Hit
GGGAGCGCCCGAATCTGGCTTGGCGGAATATCTCTTTGACAAGCACACCC307914.86793181708436No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG7033.3946593268627168No Hit
GGGGGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGC4081.970157902361292No Hit
GGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTG3761.8156357139407986No Hit
GGGGATAACTGGAGGTTGCAGGTCAATGACTGATCTTAATTGGGAAGAAC3751.8108068955526584No Hit
GGGAGAGCGGGCTGAATGCAATGGAGTGTGCATTACATTTGGAAAAAAAT2601.2554927809165097No Hit
GGGGATGCTTGCATGTGTAATCTTACTAAGAGCTAATAGAAAGGCTAGGACCAAACCATCCG2561.236177507363948No Hit
GGGACTGAACTTTGAGCTTCAGGCAGCACAACTCACATTTGTGCAAAGAG1520.7339803949973441No Hit
GGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACGA1460.7050074846685016No Hit
GGGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACG980.4732242020377614No Hit
GGGCTCACCCATCCATCCACTCATCTATCCACCCACGCATCGATCTATCC970.4683953836496209No Hit
GGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTAT970.4683953836496209No Hit
GGGGGCCTCTCCAAAGCAAGTCATTAATCTTGGTTAACGCAAGGGAATTG920.44425129170891886No Hit
GGGGAGAACAGCGTCTTTTACCTGTCACCGAAGCCAGGAAGCCCCGTTTG860.41527838138007633No Hit
GGGAGAGCGGGCTGAATGCAATGGAGTGTGCATTACATTTGGAAAAAAAA830.400791926215655No Hit
GGGGACAGAAGTGGCGCCTCTGAGAAAAGAAGGTTGGAATTATCGTAATT820.3959631078275146No Hit
GGGCAGTGGTATCAACGCAGAGTACTACTACGCCCGGTTCGCTAATTTTT680.3283596503935487Clontech SMART CDS Primer II A (100% over 24bp)
GGGGGATACAGAAGTGGCGCCTCTGAGAAAAGAAGGTTGGAATTATCGTA620.29938674006470617No Hit
GGGAACACTGTGTATCTAACTTTGGAAATGAGAAATTAACCTTTGGGACT620.29938674006470617No Hit
GGGGAAATTGAAACCTGCCTGATGTGGGATGTGCTGTGGCTGCTGCTTTG610.29455792167656575No Hit
GGGCCGATCAACAAGAAAGAGAAACTATTCAAGTATTAATGAAATTTTTA600.2897291032884253No Hit
GGGAGAAGTAACTCTTATAACTGGAGGTTGCAGGTCAATGACTGATCTTA600.2897291032884253No Hit
GGGAACTTCTGATTCCCATTTACTCCTGCAGCTTGCACCCTGCTCCATCT580.2800714665121445No Hit
GGGCAATCGAGTAGATTCCATTCCATTCCATTCCATTCCATTCCATTCCA580.2800714665121445No Hit
GGGCGAGCAGTTTTGTTGATTTAACTGTTGAGGTTTAGGGCTAAGCATAG550.26558501134772317No Hit
GGGGAGTTGATTCCATTCCATTCCATTCCATTCCATTCCATTCCATTCCA540.2607561929595828No Hit
GGGGATTGCAGACACCCAGACTGGGGACTCAGCTATCTACTTCTGTGCAG530.2559273745714424No Hit
GGGGTGACTTCCCTGGTCACCAAGGCAGTGCATGCATGTTGGGGTTTCCT510.2462697377951615No Hit
GGGATTCAGCCTCACCTCCTCTTGTTCCCTCACTCGCACAGCCAGCCCCT500.24144091940702112No Hit
GGGGAGAATGTGGAGCAGCATCCTTCAACCCTGAGTGTCCAGGAGGGAGA500.24144091940702112No Hit
GTGTGTGTTGTGTCTTGTCTGTCCTCCATAACAACTGTCCCCAGTCCTCA480.23178328263074024No Hit
GGGTCTTCGGGCCAGGGACACGGCTCACCGTGCTAGAGGACCTGAAAAAC480.23178328263074024No Hit
GGGAAAACAGCCTTACAAAGACATCCAGCTCTAAGGAGCTCAAAACATCC460.22212564585445943No Hit
GGGGTTTCCTTTACCTTTTCTATAAGTTGTACCAAACCATCCG460.22212564585445943No Hit
GGGACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAA450.21729682746631898No Hit
GGGGAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGC450.21729682746631898No Hit
GGGATACCCGATGATGGAAGTAGCTCTTATGGCTGGAGATTGCAGGTTTA430.20763919069003817No Hit
GGGATGATTCCATTCCATTCCATGCAATGATTCCATTCGATTCCGTTTGA430.20763919069003817No Hit
GGGGACTGACTAAGAAACACTGTGGGATGGATAGCAGCTATAAATTGATC420.20281037230189775No Hit
GGGGAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAA410.1979815539137573No Hit
GGGGGGAGGGATAGCATTAAGAGAAATACGCAATATAAATGACGAGTTAG410.1979815539137573No Hit
GGGCATGCAAGGCACAGGTCCTTGTGAAAAGGACAATCAAGTCTGAGACA410.1979815539137573No Hit
GGGGACTCTGCAGGGGAGCGCTGTCAGCATGACACGAGTTAGCTTGCTGT400.19315273552561688No Hit
GGGGTAGCTGTTGCTTCTGTTCTCTGGAAATTCTTTAAACCTAGAATCAG390.18832391713747645No Hit
GGGGAGTGGTAATGAAGATGGAAGGTTTACAGCACAGCTCAATAGAGCCA390.18832391713747645No Hit
GGGAGGGAAAGATAATCCCCATCCACTGCAAGGATAGTTTCCCCTAATGT370.1786662803611956No Hit
GGGGACAATGAGCAGTTCTTCGGGCCAGGGACACGGCTCACCGTGCTAGA370.1786662803611956No Hit
GGGAAGCTTCCCATCGCCCTTCCCTGCAGACCCAGCTCCCATACCTGGCC370.1786662803611956No Hit
GGGAAGAAGAAGCATGGAAGATTAATTGCCACAATAAACATACAGGAAAA360.1738374619730552No Hit
GGGTGTTGCGTGTCTGTAATCCCAGCTACTCAGCAGGCTGAGGCAGGAGA360.1738374619730552No Hit
GGGGATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTTCCCAC360.1738374619730552No Hit
GGGGCAGGGGTATATTAAAGTGACTTCATTAGATACCAGAAGATACAAGG350.16900864358491477No Hit
GGGAACCAGAAAGGACAGCTTCCTGAATATCTCAGCATCCATACCTAGTG350.16900864358491477No Hit
GGGGTCAACTGCAAAAGGCCCGCTAGCACCTAGTGCTCAGCTCACAGCCC350.16900864358491477No Hit
GGGGAGGAGGAAGCTACATACCTACATTTGGAAGAGGAACCAGCCTTATT340.16417982519677435No Hit
GGGGACCCACATGAAGTGTCTACCTTCTGCAGACTACAGTGGCTCAGGAA340.16417982519677435No Hit
GGGAGCATACGCCGAGCCGGTCTTTGAGCGCTAACGTCTTTCTGTCTCCC340.16417982519677435No Hit
GGGATTCCCCACTAACCCCCACACACACCCCCTTCCTACCAAACCATCCG340.16417982519677435No Hit
GGGGAAATAGAGTGGCAGAAGCAGCTCCTTGAGAGGGATAAACGAGAAAT330.15935100680863393No Hit
GGGGGACTCACCTTTGGCAAAGGGACTCATCTAATCATCCAGCCCTATAT330.15935100680863393No Hit
GGGAGGGTCCCCAGGGCTGTGCGAACACCGGGGAGCTGTTTTTTGGAGAA330.15935100680863393No Hit
GGGACGCATACTCATCTTTTACCGTTGGAACTTTAATAACCAAACCATCCG330.15935100680863393No Hit
GGGGGGGATGACAAGATCATCTTTGGAAAAGGGACACGACTTCATATTCT330.15935100680863393No Hit
GGGAACCAGTCTTTCAAAATCTTATTTTGGGAGAGGCCAGCTGCCATGTC320.1545221884204935No Hit
GGGGGAAGTGGATCCGCCGCTTGCGCCGCATGGAGTCCGAATCGGAAAGC320.1545221884204935No Hit
GGGAAAAGACATCCAGCTCTAAGGAGCTCAAAACATCCTGAGGACAGTGC320.1545221884204935No Hit
GGGAGTCTTTCTTCTGGTACAGACAGGATTGCAGGAAAGAACCTAAGTTG320.1545221884204935No Hit
GGGAGTCCATATAAGCGACACGGCTGAGTACTTCTGTGCTGTGAGTGTCG310.14969337003235308No Hit
GGGGAAAAAACACACTTGATAACTGAAGGATGATGAAGTGTCCACAGGCT310.14969337003235308No Hit
GGGGAAATTCAAGCTAGTACTGTAAGATTTTAACCATCAGATGGGAGCAG310.14969337003235308No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAACCAAACCATCCG310.14969337003235308No Hit
GGGGCAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCAC300.14486455164421266No Hit
GCATGTTGGGGTTTCCTTTACCTTTTCTATAAGTTGTACCAAACCATCCG300.14486455164421266No Hit
GACAGACACCAAGGCCCACTTGAGGGTGAAGGATGGGAGGATGCTGAGGA290.14003573325607224No Hit
GGGGGACGCTGTGTGGAATATGGAAACAAGCTGGTCTTTGGCGCAGGAAC290.14003573325607224No Hit
GGATATCCCATTCTCCATGACGTGATTATTTCACATTTCATGCTTGTACCAAACCATCCG290.14003573325607224No Hit
GGGAATCTCCCAAGGCAGAGTCCTTCCAGCTTTCCTCCCCCTCAACTTCA280.13520691486793182No Hit
GGGTCAATAAAAAAAATTAAGAAGGAATTTAAAATTTCTTGAAACAAAAG280.13520691486793182No Hit
GGGCTAGCACTCTGACCTAGCAGTCAACATGAAGGCTCTCATTGTTCTGG280.13520691486793182No Hit
GGGGAGTGGAGTGTGTGGTGGGGCCCCAAGAGTTCTGTGTGTAAGCAGCT280.13520691486793182No Hit
GGGTAAAAACAGCCAGCATGTGAGGCTAGAAGCAAGTTGGAATCAGCCAT280.13520691486793182No Hit
GGGGGAGCTGTGATGAGAACAAGAGGTCAGAACACATCCAGGCTCCTTAA280.13520691486793182No Hit
GGGAAAAACATTGAAGACGTCATTGCCCAGGGTATTGGCAAGCTTGCCAGTGTACCAGCTGAGAGAC270.1303780964797914No Hit
GGGATTAGTGCCCTTATAAGAAGATGTCAGAGAGCTAACTTCCTCTTTTT270.1303780964797914No Hit
GGGGAGTTCTCAGTGAGTCTAAGTGACAGAAGGAATGGAGACCCTCTTGG260.12554927809165098No Hit
GGGAATGAAAGGAGTCATCATCCAATGCAATTCCATTAGATGATGACTCC260.12554927809165098No Hit
GGGGCAAGATTAGAGATACGAGGTGGAGGGATCATGCAGGGCTTTGCTCA260.12554927809165098No Hit
GGGGGGAGACATCCTCTCTAGCCCCAACTGTGCCATGACTATCAGGCTCC260.12554927809165098No Hit
GGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTT260.12554927809165098No Hit
GGGTCACATTGGAAGACAGAACTGTCTTGGGCCACACATAAAATACACTA260.12554927809165098No Hit
GGGAGTTCTTCGGGCCAGGGACACGGCTCACCGTGCTAGAGGACCTGAAA240.11589164131537012No Hit
GGGGAGATTGGCTCCTGTAGCAAAGGATGGGGAGTAGAGGGGTTCTCTTA240.11589164131537012No Hit
GGGCAGGCATCTCAGCCCCTGCCTGTATTTCCAGAGCCCCCAGGCTTCCC230.11106282292722972No Hit
GGGAAAAAAAGCAGCTAAACCAAAAGAAGCCTCCAGACAGCCCTGAGATC230.11106282292722972No Hit
GGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACAGTGC230.11106282292722972No Hit
GGGGCTTTCAACTGTACTTACAGCAACAGTGCTTCTCAGTCTTTCTTCTG230.11106282292722972No Hit
GGGAAGGCTAGTGGGCTCTGCAGGGGTAGTTGTGAGGACTAACTATTTAA220.10623400453908928No Hit
GGGAGAGGAGTTTATGTAAAGGGTTGGCCCAGAGTGTGTATTCAGGAGGA220.10623400453908928No Hit
GGGGAATTCTTCTCTCACCATGCCAGGTTCACCTCACAGTACAGAGTCCT220.10623400453908928No Hit
GGGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGTCT220.10623400453908928No Hit
GTGAATCTCCAGTCAGGCCTAACCTATGTTTTTACCAAACCATCCG210.10140518615094887No Hit
GGGGCTTCCCCAGGAGGACCAAGCCCTGAATCAGGTGCAGTGCTGCCTGC210.10140518615094887No Hit
GGGTATTAAGCCCAACATCCTGATGATCTCCCTCCCCACAATCCTCCCCA210.10140518615094887No Hit
GGGAGGAGGAAGCTACATACCTACATTTGGAAGAGGAACCAGCCTTATTG210.10140518615094887No Hit
GGGGAACAATGACATGCGCTTTGGAGCAGGGACCAGACTGACAGTAAAAC210.10140518615094887No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)