FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004953555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20709
Sequences flagged as poor quality0
Sequence length290-300
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTCTGANNGNTCACTCTGCNTNGATANCACTGCTTCCGCGGACAGGC5482.6461924767009513No Hit
CGGATGGTTNNGNACAACTTATNGNAAAGNTAAAGGAAACCCCAACATGC3661.7673475300593944No Hit
TGCTNCNGANNGNTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC2611.2603215993046502No Hit
CTTGGGGGANNCNCTCTGCGTTNANACCANTGCTTCCGCGGACAGGCTAA2551.2313486889758076No Hit
TGCNTCTGANNGNTCACTCTGCNNNGNTANCACTGCTTCCGCGGACAGGC2171.0478535902264714No Hit
TGCTTCTGANNGNTCACTCTGCGNNGNTACCACTGCTTCCGCGGACAGGC2131.02853831667391No Hit
TGCTNCNGANNGNTCACTCTGCGNTGNTACCACTNCTTCCGCGGACAGGC2131.02853831667391No Hit
TGCTTCTGANNGNTCACTCTGCNNNGNTANNACTGCTTCCGCGGACAGGC2111.018880679897629No Hit
TGCTTCTGANNGNTCACTCTGCNTNGNTANNACTGCTTCCGCGGACAGGC2111.018880679897629No Hit
CGGANGNTTNNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC1840.8885025834178377No Hit
TGCTTCNGANNGNTCACTCTGCGNTGNTANCACTGCTTCCGCGGACAGGC1810.8740161282534165No Hit
CGGANGNTTNNGNACAACTTATANAANAGGTAAANGAAACCCCAACATGC1730.835385581148293No Hit
CGGATGGTTNNGNACAACTTATNNNANAGNNAAAGGAAACCCCAACATGC1640.7919262156550292No Hit
TGCTTCTGANNGNTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC1640.7919262156550292No Hit
CGGNTGGTTNNGNACAACTTATNNNANAGNTAAAGGAAACCCCAACATGC1560.7532956685499058No Hit
CGGATGNTTNNGNACAACTTATANAANAGNTAAAGGAAACCCCAACATGC1310.6325752088463953No Hit
CTTGNGNGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCTAA1230.5939446617412719No Hit
CGGATGGTTNNGNACAACTTATNGNANAGNNAAAGGAAACCCCAACATGC1230.5939446617412719No Hit
TGCNTCTGANNGNTCACTCTGCNNNGNTANNACTGCTTCCGCGGACAGGC1200.5794582065768507No Hit
CGGATGGTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC1170.5649717514124294No Hit
CTTGGGGGANNCNCTCTGCGTTNNNANCANNGCTTCCGCGGACAGGCTAA1140.5504852962480081No Hit
TGCTTCNGANNGNTCACTCTGCGNNGNTACCACTGCTTCCGCGGACAGGC1130.5456564778598677No Hit
TGCTTCTGANNGNTCACTCTGCNTNGATANNACTGCTTCCGCGGACAGGC1130.5456564778598677No Hit
TGCNTCTGANNGNTCACTCTGCNTNGATANCACTGCTTCCGCGGACAGGC1090.526341204307306No Hit
TGCNTCTGANNGNTCACTCTGCNNNGNTACCACTGCTTCCGCGGACAGGC1080.5215123859191656No Hit
CGGATGGTTNNGNTAAGGTTGTNTNGTAGNAAGGTGGAGTGGGTTTGGGG1080.5215123859191656No Hit
TGCTNCNGANNGCTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC1070.5166835675310252No Hit
TGCNTCTGANNGNTCACTCTGCNTNGATANNACTGCTTCCGCGGACAGGC1070.5166835675310252No Hit
CGGATGGTTNNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC1060.5118547491428848No Hit
TGCTTCNGANNGCTCACTCTGCGNNGNTACCACTGCTTCCGCGGACAGGC1060.5118547491428848No Hit
TGCTTCTGANNGNTCACTCTGCNTTGATANCACTGCTTCCGCGGACAGGC1050.5070259307547443No Hit
TGCTNCNGANNGNTCACTCTGCGNNGNTANCACTGCTTCCGCGGACAGGC1030.4973682939784635No Hit
TGCTTCTGANNGNTCACTCTGCGTNGATANCACTGCTTCCGCGGACAGGC1020.492539475590323No Hit
TGCTTCTGANNGNTCACTCTGCGNNGNTANCACTNCTTCCGCGGACAGGC1010.48771065720218265No Hit
TGCTTCTGANNGNTCACTCTGCNTNGNTANCACTGCTTCCGCGGACAGGC1000.48288183881404223No Hit
CTTGGGNGANNCNCTCTGCGTTGNTANCANTGCTTCCGCGGACAGGCTAA1000.48288183881404223No Hit
CTTGGGGGANNCNCTCTGCGTTNANANCANNGCTTCCGCGGACAGGCTAA950.4587377468733401No Hit
CTTGNGNGANNCNCTCTGCGTTGNTANCACTGCTNCCGCGGACAGGCTAA940.45390892848519965No Hit
CGGANGNTTNNGNACAACTTATANNANAGNTAAAGGAAACCCCAACATGC910.4394224733207784No Hit
CGGATGGTTNNGNACAACTTATANNANAGNTAAANGAAACCCCAACATGC890.4297648365444976No Hit
TGCTNCTGANNGNTCACTCTGCGNNGNTACCACTGCTTCCGCGGACAGGC890.4297648365444976No Hit
CGGANGNTTNNGTACAACTTATANAANAGGTAAAGGAAACCCCAACATGC860.41527838138007633No Hit
CGGANGNTTNNGNACAACTTATANNANAGNTAAANGAAACCCCAACATGC830.400791926215655No Hit
CGGATGGTTNNGNACAACTTATNGCAAAGNTAAAGGAAACCCCAACATGC830.400791926215655No Hit
TGCTTCNGANNGNTCACTCTGCNNNGNTACCACTNCTTCCGCGGACAGGC820.3959631078275146No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACCTTGTTCA820.3959631078275146No Hit
CGGATGNTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC820.3959631078275146No Hit
CGGNTGGTTNNGNACAACTTATNGNAAAGNTAAAGGAAACCCCAACATGC800.38630547105123375No Hit
CGGNTGGTTNNGNACAACTTATNNNANAGNNAAAGGAAACCCCAACATGC790.38147665266309333No Hit
TGCTNCNGANNGNTCACTCTGCGNNGNTANCACTNCTTCCGCGGACAGGC790.38147665266309333No Hit
CGGATGGTTNNGNACAACTTATNGNAAAGNNAAAGGAAACCCCAACATGC770.3718190158868125No Hit
TGCTNCNGATNGNTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC760.36699019749867207No Hit
CTTNGGGGANNCNCTCTGCGTTNNNANCANTGCTTCCGCGGACAGGCTAA730.3525037423342508No Hit
CGGATGGTTNNGNACAACTTATCGNAAAGNTAAAGGAAACCCCAACATGC700.33801728716982954No Hit
CGGATGNTTNNGNACAACTTATNNNANAGGTAAANGAAACCCCAACATGC690.3331884687816891No Hit
CTTGGGGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTAA670.3235308320054083No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACGTTTTTCA670.3235308320054083No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGATGGA630.3042155584528466No Hit
CGGNTGGTTNNGNACAACTTATNGNAAAGNNAAAGGAAACCCCAACATGC600.2897291032884253No Hit
CGGNTGGTTNNGNACAACTTATNNNANAGGTAAAGGAAACCCCAACATGC600.2897291032884253No Hit
CTTNGGGGANNCNCTCTGCGTTNANACCANTGCTTCCGCGGACAGGCTAA590.2849002849002849No Hit
CGGANGNTTTNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC580.2800714665121445No Hit
CGGATGGTTNNGNACAACTTATNGNANAGNTAAAGGAAACCCCAACATGC580.2800714665121445No Hit
CTTGGGGGANNCNCTCTGCGTTGNNANCANTGCTNCCGCGGACAGGCTAA570.27524264812400406No Hit
CTTGGGGGANNCNCTCTGCGTTGANACCANTGCTTCCGCGGACAGGCTAA530.2559273745714424No Hit
CGGATGNTTNNGTACAACTTATANNANAGGTAAAGGAAACCCCAACATGC530.2559273745714424No Hit
CGGANGGTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC510.2462697377951615No Hit
CTTGGGNGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTAA510.2462697377951615No Hit
CTTGNGNGANNCCCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCTAA500.24144091940702112No Hit
CTTGGGGGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCTAA480.23178328263074024No Hit
CTTGGGGGANNCNCTCTGCGTTNANACCANNGCTTCCGCGGACAGGCTAA470.22695446424259982No Hit
CTTGGTGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTAA450.21729682746631898No Hit
CTTNGGGGANNCNCTCTGCGTTNNNANCANNGCTTCCGCGGACAGGCTAA450.21729682746631898No Hit
CTTGNGNGANNCNCTCTGCGTTGNNANCANTGCTTCCGCGGACAGGCTAA450.21729682746631898No Hit
CTTGNGNGANNCNCTCTGCGTTGNNANCANTGCTNCCGCGGACAGGCTAA440.21246800907817856No Hit
CGGANGNTTNNGNTAAGGTTGTCNGGNAGTAAGGTGGAGTGGGTTTGGGG430.20763919069003817No Hit
CGGATGGTTNNGNTAAGGTTGTNTNGNAGNNAGGTGGAGTGGGTTTGGGG420.20281037230189775No Hit
CTTGGGGGANNCNCTCTGCGTTNATACCANTGCTTCCGCGGACAGGCTAA400.19315273552561688No Hit
CGGATGGTTNNGNTAAGGTTGTNNNGNAGNNAGGTGGAGTGGGTTTGGGG390.18832391713747645No Hit
CTTGGGGGANNCNCTCTGCGTTNANANCANTGCTTCCGCGGACAGGCTAA380.18349509874933603No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTNGGAACACCTTGTTCA380.18349509874933603No Hit
CTTGGTGGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCTAA360.1738374619730552No Hit
CGGATGGTTNNGNCCTAGCCTTNCNATTANCTCTTAGTAAGATTACACAT350.16900864358491477No Hit
CGGATGNTTNNGNTAAGGTTGTCNGGNAGNAAGGTGGAGTGGGTTTGGGG340.16417982519677435No Hit
TGCTTCNGANNGNTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC340.16417982519677435No Hit
CTTGGGGGANNCNTCACCCGGCNCNGACANGGACAGGATTGACAGATTGA340.16417982519677435No Hit
CGGATGGTTNNGNTGGTGTCGTNTNGTCCNGAGAGTCCAGTTTGTATCTG330.15935100680863393No Hit
CTTGGGNGANNCNCTCTGCGTTNNNANCACTGCTNCCGCGGACAGGCTAA330.15935100680863393No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNGGGTGGGAACACCTTGTTCA320.1545221884204935No Hit
CGGNTGGTTNNGNTAAGGTTGTNNNGNAGNAAGGTGGAGTGGGTTTGGGG320.1545221884204935No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA320.1545221884204935No Hit
CGGANGNTTNNGNTAAGGTTGTCNGGNAGTAAGGNGGAGTGGGTTTGGGG310.14969337003235308No Hit
CTTNGGGGANNCNCTCTGCGTTNANACCANNGCTTCCGCGGACAGGCTAA310.14969337003235308No Hit
CGGATTGTTNNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC300.14486455164421266No Hit
CGGANTGTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC300.14486455164421266No Hit
CTTNGGGGANNCNCTCTGCGTTNNNANCACTGCTTCCGCGGACAGGCTAA300.14486455164421266No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATTTGGATGGA290.14003573325607224No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATTTGGATGGA290.14003573325607224No Hit
TGCTTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACCTTGTTCA290.14003573325607224No Hit
CGGATTNTTNNGTACAACTTATANNANAGGTAAAGGAAACCCCAACATGC290.14003573325607224No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA280.13520691486793182No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACCTTGTTCA270.1303780964797914No Hit
CTTGNGNGACNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCTAA270.1303780964797914No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATTTGGATGGA270.1303780964797914No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTNGGAACACGTTTTTCA260.12554927809165098No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNNGGTGGGAACACCTTGTTCA260.12554927809165098No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA250.12072045970351056No Hit
CTTGGGGGANNCNTCTGCATCANTNTGCGNTGATACCACTGCTTCCGCGG250.12072045970351056No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATTTGGATGGA240.11589164131537012No Hit
CGGATGGTTNNGNTAAGGTTGTCNNGNAGTAAGGTGGAGTGGGTTTGGGG240.11589164131537012No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATTTGGATGGA240.11589164131537012No Hit
CTTNGTGGANNCNCTCTGCGTTNNNANCANTGCTTCCGCGGACAGGCTAA230.11106282292722972No Hit
CGGNTGGTTNNGNTAAGGTTGTNTNGTAGNAAGGTGGAGTGGGTTTGGGG230.11106282292722972No Hit
CTTGGGNGANNCCCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTAA220.10623400453908928No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTNGGGTGGGAACACCTTGTTCA220.10623400453908928No Hit
CGGATGGTTNNGNATAACTTATNGNAAAGNTAAAGGAAACCCCAACATGC220.10623400453908928No Hit
CTTGGTNGANNCCCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTAA220.10623400453908928No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTGGGAACACCTTGTTCA220.10623400453908928No Hit
CGGATGGTTNNGNTAAGGTTGTNCNCATTNACTCTGCGTTGATACCACTG210.10140518615094887Clontech SMART CDS Primer II A (95% over 21bp)
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATTTGGATGGA210.10140518615094887No Hit
CTTGNGGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTAA210.10140518615094887No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA210.10140518615094887No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNGGGTGGGAACACGTTTTTCA210.10140518615094887No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNGGGTGGGAACACGTTTTTCA210.10140518615094887No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTNGGGTNGGAACACCTTGTTCA210.10140518615094887No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)