FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004953559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38838
Sequences flagged as poor quality0
Sequence length294-300
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACGTTTTTCA5691.4650599927905659No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACCTTGTTCA3830.986147587414388No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTGGGTTTCA3370.8677068850095269No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA2590.666872650496936No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTNGGGTGGGAACACGTTTTTCA2510.6462742674700036No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA2390.6153766929296051No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTNGGAACACGTTTTTCA2340.6025027035377722No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNNGGTGGGAACACGTTTTTCA2240.5767547247541068No Hit
TGCTTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACGTTTTTCA2210.5690303311190071No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNGGGTGGGAACACGTTTTTCA2110.5432823523353417No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCAGGCCTGA2010.5175343735516762No Hit
TGCTTCTGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA1970.50723518203821No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATACGGATGAA1620.4171172562953808No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTNGGAACACCTTGTTCA1560.40166846902518155No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNGGGTGGGAACACCTTGTTCA1540.39651887326844837No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA1540.39651887326844837No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA1540.39651887326844837No Hit
TGCTTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACCTTGTTCA1540.39651887326844837No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTGGGTTTCA1510.3887944796333488No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNNGGTGGGAACACCTTGTTCA1500.3862196817549822No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTNGGGTGGGAACACCTTGTTCA1420.3656212987280498No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATAGGGCTGGA1360.35017251145785055No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATTGGGTTTCA1280.32957412843091816No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACGTTTTTCA1240.319274936917452No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATTGGGTTTCA1240.319274936917452No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGTTTTA1240.319274936917452No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATTGGGTTTCA1230.31670013903908545No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTNGGAACACGTTTTTCA1230.31670013903908545No Hit
CGGATGGTTNNGNACAACTTATNGNAAAGNTAAAGGAAACCCCAACATGC1220.3141253411607189No Hit
TGCTTCNGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA1210.3115505432823523No Hit
TGCTTCTGANNGNTCAAACACAGCNACCTNGGGTGGGAACACGTTTTTCA1200.3089757454039858No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATTGGGTTTCA1200.3089757454039858No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACGTTTTTCA1190.30640094752561925No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTNGGGTNGGAACACGTTTTTCA1150.29610175601215305No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACGTTTTTCA1140.2935269581337865No Hit
TGCTNCNGANNGCTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA1130.29095216025542No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNGGGTGGGAACACGTTTTTCA1120.2883773623770534No Hit
TGCTNCNGATNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA1120.2883773623770534No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTGGGAACACGTTTTTCA1120.2883773623770534No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATTGGGTTTCA1070.27550337298522065No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACGTTTTTCA1070.27550337298522065No Hit
TGCTNCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA1070.27550337298522065No Hit
TGCTTCTGANNGNTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA1060.27292857510685414No Hit
TGCNTCTGANNGNTCAAACACANNNANCTCGGGTGGGAACACGTTTTTCA1040.267778979350121No Hit
TGCTTCNGANNGCTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA1030.26520418147175445No Hit
TGCTTCTGANNGNTCAAACACANCGACCTNGGGTGGGAACACGTTTTTCA1010.2600545857150214No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACCTTGTTCA950.24460579844482205No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCAGGTAAAA940.24203100056645555No Hit
TGCTTCNGANNGNTCAAACACANNNANCTCGGGTNGGAACACGTTTTTCA940.24203100056645555No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATTGGGTTTCA940.24203100056645555No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGAATGA930.23945620268808898No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATAGGACTTGA910.23430660693135588No Hit
GTCTCTCAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTGGGTTTCA910.23430660693135588No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATCAGGCCTGA880.22658221329625627No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATCAGGCCTGA870.2240074154178897No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTNGGAACACCTTGTTCA860.22143261753952312No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATCAGGCCTGA830.2137082239044235No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCGAGCGTGA830.2137082239044235No Hit
TGCNTCTGANNGNTCAAACACANNNANCTCGGGTGGGAACACCTTGTTCA800.20598383026932385No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCAGGCCAGA790.20340903239095728No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNNTTCTGGATATTGGGTTTCA770.19825943663422418No Hit
TGCTTCTGANNGNTCAAACACAGCNACCTNGGGTGGGAACACCTTGTTCA750.1931098408774911No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGCTTCA750.1931098408774911No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATCAGGCCTGA740.19053504299912455No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACCTTGTTCA740.19053504299912455No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGTTTAA740.19053504299912455No Hit
GTCTCTNAGNNGGTACACGGCAGNNTNAGGGTTCTGGATATTGGGTTTCA730.187960245120758No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACCTTGTTCA720.18538544724239148No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNGTTCTGGATATTGGGTTTCA720.18538544724239148No Hit
TGCTTCTGANNGNTCAAACACANCGACCTNGGGTGGGAACACCTTGTTCA710.1828106493640249No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTNGGGTNGGAACACCTTGTTCA710.1828106493640249No Hit
TGCTTCNGANNGNTCAAACACANNNANCTCGGGTNGGAACACCTTGTTCA700.18023585148565838No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATACGGATGAA700.18023585148565838No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTGGGAACACCTTGTTCA700.18023585148565838No Hit
GTCTNTNAGNNGNTACACGGCAGNNTNAGNGTTCNGGATATTGGGTTTCA690.17766105360729184No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATACGGATGAA690.17766105360729184No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACCTTGTTCA690.17766105360729184No Hit
TGCTTCNGANNGNTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA690.17766105360729184No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNGGGTGGGAACACCTTGTTCA690.17766105360729184No Hit
TGCTTCNGANNGCTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA690.17766105360729184No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATACGGATGAA680.17508625572892528No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATCAGGCCTGA680.17508625572892528No Hit
TGCTNCNGATNGNTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA670.1725114578505587No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATCAGGCCTGA670.1725114578505587No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATCAGGCCTGA650.16736186209382564No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATACGGATGAA650.16736186209382564No Hit
TGCTNCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA640.16478706421545908No Hit
GTCTCTNAGNNGNTACACGGCANNNTNAGGGTTCNGGATATTGGGTTTCA630.16221226633709254No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGCTAAAA630.16221226633709254No Hit
GTCTNTNAGNNGGTACACGGCAGNGTNAGGGTTCTGGATATTGGGTTTCA620.159637468458726No Hit
CGGANGNTTNNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC620.159637468458726No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATACGGATGAA620.159637468458726No Hit
TGCTNCNGANNGCTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA620.159637468458726No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGGAATGA610.15706267058035944No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATTGGGTTTCA610.15706267058035944No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGCTTTAA600.1544878727019929No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGATGGA590.15191307482362634No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTGGGTATGA590.15191307482362634No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATAGGGCTGGA590.15191307482362634No Hit
GTCTNTNAGNNGNTACACGGCAGNNTNAGNGTTCTGGATATTGGGTTTCA580.14933827694525978No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATAGGGCTGGA570.14676347906689324No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATAGGGCTGGA550.14161388331016014No Hit
CGGATGGTTNNGNACAACTTATNNNANAGNNAAAGGAAACCCCAACATGC550.14161388331016014No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATACGGATGAA540.1390390854317936No Hit
GTCNCTCAGNNGNTACACGGCANGNTCAGNNTTCTGGATATTGGGTTTCA530.13646428755342707No Hit
GTCTCTCAGNNGNTACACGGCAGGNTCAGNGTTCTGGATATTGGGTTTCA530.13646428755342707No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATAGGGCTGGA530.13646428755342707No Hit
GTCTCTCAGNNGNTACACGGCANGGTCAGNGTTCTGGATATTGGGTTTCA530.13646428755342707No Hit
GTCTCTNAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATTGGGTTTCA520.1338894896750605No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATAGGGCTGGA520.1338894896750605No Hit
CGGANGNTTNNGNACAACTTATANAANAGGTAAANGAAACCCCAACATGC510.13131469179669394No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATTTGGTTTTA510.13131469179669394No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGTATGA510.13131469179669394No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGNGTTCNGGATATTGGGTTTCA500.1287398939183274No Hit
GTCNCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTGGGTTTCA500.1287398939183274No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATAGGGCTGGA500.1287398939183274No Hit
GTCTCTCAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATACGGATGAA500.1287398939183274No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATACGGATGAA490.12616509603996087No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGAAAGA490.12616509603996087No Hit
CGGATGGTTNNGNACAACTTATNGNANAGNNAAAGGAAACCCCAACATGC490.12616509603996087No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATTTGGAATGA480.12359029816159431No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGGGTTCTGGATATTGGGTTTCA480.12359029816159431No Hit
GTCTNTNAGCNGNTACACGGCAGNGTNAGGGTTCTGGATATTGGGTTTCA470.12101550028322777No Hit
GTCTNTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATTGGGTTTCA470.12101550028322777No Hit
CGGATGNTTNNGNACAACTTATANAANAGNTAAAGGAAACCCCAACATGC460.11844070240486122No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATCAGGTAAAA450.11586590452649467No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATTTGGTTTTA450.11586590452649467No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATTTGGTTTAA450.11586590452649467No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATAGGGCAGCA440.11329110664812814No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGCTCTTA440.11329110664812814No Hit
GTCTCTNAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATCAGGCCTGA440.11329110664812814No Hit
CGGNTGGTTNNGNACAACTTATNNNANAGNTAAAGGAAACCCCAACATGC440.11329110664812814No Hit
GTCTCTCAGNNGNTACACGGCAGGNTCAGNGTTCTGGATATCAGGCCTGA440.11329110664812814No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA440.11329110664812814No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCTGGGGTGA430.11071630876976156No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCTGGTTTTA430.11071630876976156No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATTTGGTTTTA430.11071630876976156No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATGGGGTTTGA420.10814151089139502No Hit
CGGATGGTTNNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC420.10814151089139502No Hit
GTCTNTNAGNNGNTACACGGCAGNNTNAGNGTTCTGGATATCAGGCCTGA420.10814151089139502No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATTTGGTTTTA420.10814151089139502No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGACTGA420.10814151089139502No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATTTGGATGGA410.10556671301302847No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATCAGGTAAAA410.10556671301302847No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATAGGGCTGGA410.10556671301302847No Hit
GTCTCTCAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATCAGGCCTGA410.10556671301302847No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCTGCTGCGT410.10556671301302847No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATTTGGTTTTA410.10556671301302847No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCTGGCTTTA410.10556671301302847No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATCAGGCCAGA400.10299191513466192No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATTTGGAATGA390.10041711725629539No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATTTGGAATGA390.10041711725629539No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)