FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004953563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169288
Sequences flagged as poor quality0
Sequence length289-300
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACGTTTTTCA35992.125962856197722No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA16550.9776239308161241No Hit
CGGATGGTTNNGNACAACTTATNGNAAAGNTAAAGGAAACCCCAACATGC16180.9557676858371533No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA15150.8949246254902888No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNGGGTGGGAACACGTTTTTCA14690.8677519965975143No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTNGGAACACGTTTTTCA14660.8659798686262463No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTNGGGTGGGAACACGTTTTTCA14230.84057936770474No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNNGGTGGGAACACGTTTTTCA14120.8340815651434244No Hit
TGCTTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACGTTTTTCA13900.8210859600207929No Hit
TGCTTCTGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA11580.6840413969094088No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTGGGTTTCA9580.5658995321582155No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACCTTGTTCA8620.5091914370776428No Hit
CGGANGNTTNNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC7910.46725107509096925No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTNGGAACACGTTTTTCA7740.45720901658711777No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTGGGAACACGTTTTTCA7730.45661830726336183No Hit
TGCTTCNGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA7590.4483483767307783No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACGTTTTTCA7540.4453948301119985No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACGTTTTTCA7470.4412598648457067No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNGGGTGGGAACACGTTTTTCA7400.43712489957941497No Hit
TGCTNCNGANNGCTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA7150.42235716648551574No Hit
CGGANGNTTNNGNACAACTTATANAANAGGTAAANGAAACCCCAACATGC7090.41881291054298No Hit
TGCTTCNGANNGCTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA6950.41054298001039646No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACGTTTTTCA6870.4058173054203487No Hit
TGCTTCTGANNGNTCAAACACAGCNACCTNGGGTGGGAACACGTTTTTCA6790.401091630830301No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACGTTTTTCA6700.39577524691649735No Hit
TGCTTCNGANNGNTCAAACACANNNANCTCGGGTNGGAACACGTTTTTCA6690.3951845375927414No Hit
TGCTTCTGANNGNTCAAACACANCGACCTNGGGTGGGAACACGTTTTTCA6660.3934124096214735No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTNGGGTNGGAACACGTTTTTCA6540.3863238977364019No Hit
TGCTNCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA6480.3827796417938661No Hit
CGGATGGTTNNGNACAACTTATNNNANAGNNAAAGGAAACCCCAACATGC6450.3810075138225982No Hit
CGGATGGTTNNGNACAACTTATNGNANAGNNAAAGGAAACCCCAACATGC6360.3756911299087945No Hit
CGGATGNTTNNGNACAACTTATANAANAGNTAAAGGAAACCCCAACATGC6100.3603326874911394No Hit
TGCNTCTGANNGNTCAAACACANNNANCTCGGGTGGGAACACGTTTTTCA6090.3597419781673834No Hit
TGCTNCNGATNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA5960.3520627569585559No Hit
CGGNTGGTTNNGNACAACTTATNNNANAGNTAAAGGAAACCCCAACATGC5840.34497424507348423No Hit
CGGATGGTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC5800.3426114077784604No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATACGGATGAA5330.31484806956192996No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGAATGA4960.2929918245829592No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATAGGGCTGGA4780.28235905675535183No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTGGGTTTCA4250.25105146259628563No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGTTTAA4220.2492793346250177No Hit
CGGATGGTTNNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC4190.2475072066537498No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCAGGTAAAA4180.24691649732999388No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA3960.2339208922073626No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTNGGAACACCTTGTTCA3860.22801379896980295No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATTGGGTTTCA3850.22742308964604696No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATTGGGTTTCA3820.22565096167477908No Hit
CGGATGNTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC3740.22092528708473136No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA3560.21029251925712397No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATTGGGTTTCA3500.20674826331458815No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATTGGGTTTCA3490.2061575539908322No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCAGGCCTGA3470.20497613534332026No Hit
CGGATGGTTNNGNACAACTTATANNANAGNTAAANGAAACCCCAACATGC3450.20379471669580831No Hit
CGGANGNTTNNGNACAACTTATANNANAGNTAAANGAAACCCCAACATGC3430.2026132980482964No Hit
CGGANGNTTNNGTACAACTTATANAANAGGTAAAGGAAACCCCAACATGC3400.2008411700770285No Hit
CGGATGGTTNNGNACAACTTATNGNANAGNTAAAGGAAACCCCAACATGC3390.20025046075327252No Hit
TGCTTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACCTTGTTCA3380.19965975142951656No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTNGGGTGGGAACACCTTGTTCA3360.19847833278200464No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATTGGGTTTCA3310.1955247861632248No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNNGGTGGGAACACCTTGTTCA3300.19493407683946884No Hit
CGGNTGGTTNNGNACAACTTATNGNAAAGNTAAAGGAAACCCCAACATGC3270.19316194886820096No Hit
CGGANGNTTNNGNACAACTTATANNANAGNTAAAGGAAACCCCAACATGC3220.1902084022494211No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATTGGGTTTCA3210.18961769292566513No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNGGGTGGGAACACCTTGTTCA3200.18902698360190917No Hit
CGGNTGGTTNNGNACAACTTATNNNANAGNNAAAGGAAACCCCAACATGC3190.1884362742781532No Hit
CGGATGNTTNNGNACAACTTATNNNANAGGTAAANGAAACCCCAACATGC3080.18193847171683758No Hit
GTCTCTCAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTGGGTTTCA3080.18193847171683758No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGATGGA3060.18075705306932563No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGTTTTA3050.1801663437455697No Hit
CGGATGGTTNNGNACAACTTATNGNAAAGNNAAAGGAAACCCCAACATGC3030.17898492509805775No Hit
CGGNTGGTTNNGNACAACTTATNGNAAAGNNAAAGGAAACCCCAACATGC2970.17544066915552195No Hit
CGGATGGTTNNGNACAACTTATCGNAAAGNTAAAGGAAACCCCAACATGC2890.17071499456547423No Hit
TGCTTCTGANNGNTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA2790.16480790132791456No Hit
CGGNTGGTTNNGNACAACTTATNNNANAGGTAAAGGAAACCCCAACATGC2740.16185435470913473No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCGAGCGTGA2720.1606729360616228No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA2650.15653797079533102No Hit
CGGANGNTTTNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC2640.15594726147157506No Hit
CGGATGGTTNNGNACAACTTATNGCAAAGNTAAAGGAAACCCCAACATGC2610.15417513350030718No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGGAATGA2510.1482680402627475No Hit
CGGATGNTTNNGTACAACTTATANNANAGGTAAAGGAAACCCCAACATGC2480.1464959122914796No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATACGGATGAA2410.14236094702518784No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTGGGGAGAA2310.1364538537876282No Hit
CGGANGGTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC2280.1346817258163603No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATACGGATGAA2220.1311374698738245No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGCTGGA2200.12995605122631254No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATAGGGCTGGA2180.12877463257880062No Hit
CGGATGGTTNNGNTAAGGTTGTNTNGTAGNAAGGTGGAGTGGGTTTGGGG2160.1275932139312887No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATTTGGAATGA2150.12700250460753273No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATAGGGCTGGA2140.12641179528377677No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGTATGA2130.12582108596002078No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGAAAGA2130.12582108596002078No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGCTTTAA2070.122276830017485No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATAGGGCTGGA2060.12168612069372903No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATAGGGCTGGA2050.12109541136997307No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATACGGATGAA2050.12109541136997307No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATACGGATGAA2040.12050470204621709No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATACGGATGAA2040.12050470204621709No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATTTGGAATGA2030.11991399272246112No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATACGGATGAA2010.11873257407494921No Hit
GTCTNTNAGNNGGTACACGGCAGNGTNAGGGTTCTGGATATTGGGTTTCA2000.11814186475119323No Hit
GTCTCTNAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATTGGGTTTCA1990.11755115542743727No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNGTTCTGGATATTGGGTTTCA1970.11636973677992533No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATAGGGCTGGA1970.11636973677992533No Hit
GTCTNTNAGNNGNTACACGGCAGNNTNAGNGTTCNGGATATTGGGTTTCA1960.11577902745616936No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTGGGAACACCTTGTTCA1950.11518831813241341No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGCTAAAA1940.11459760880865744No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGCAATCA1940.11459760880865744No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATTTGGAATGA1930.11400689948490147No Hit
TGCTTCTGANNGNTCAAACACAGCNACCTNGGGTGGGAACACCTTGTTCA1930.11400689948490147No Hit
GTCNCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTGGGTTTCA1920.1134161901611455No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNNTTCTGGATATTGGGTTTCA1920.1134161901611455No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATTTGGAATGA1910.11282548083738954No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATTTGGAATGA1910.11282548083738954No Hit
CGGATGGTTNNGNTGGTGTCGTNTNGTCCNGAGAGTCCAGTTTGTATCTG1900.11223477151363356No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATTTGGAATGA1900.11223477151363356No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGGTTTAA1900.11223477151363356No Hit
TGCTTCNGANNGNTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA1900.11223477151363356No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATTTGGTTTAA1900.11223477151363356No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATCAGGTAAAA1890.1116440621898776No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACCTTGTTCA1880.11105335286612163No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACCTTGTTCA1860.10987193421860972No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATACGGATGAA1860.10987193421860972No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATAGGGCTGGA1850.10928122489485374No Hit
GTCTNTNAGNNGNTACACGGCAGNNTNAGNGTTCTGGATATTGGGTTTCA1830.1080998062473418No Hit
GTCTCTCAGNNGNTACACGGCANGGTCAGNGTTCTGGATATTGGGTTTCA1830.1080998062473418No Hit
TGCTTCNGANNGCTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA1830.1080998062473418No Hit
GTCTCTNAGNNGGTACACGGCAGNNTNAGGGTTCTGGATATTGGGTTTCA1820.10750909692358583No Hit
TGCTNCNGANNGCTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA1820.10750909692358583No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCTGGTTTTA1800.10632767827607392No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATCAGGCCTGA1800.10632767827607392No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTNGGGTNGGAACACCTTGTTCA1770.10455555030480601No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATTTGGTTTAA1770.10455555030480601No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNGGGTGGGAACACCTTGTTCA1770.10455555030480601No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATTTGGTTTAA1760.10396484098105005No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATAGGGCTGGA1730.10219271300978215No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATTGGGTTTCA1730.10219271300978215No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGGATGGA1710.10101129436227022No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)