FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004953581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163634
Sequences flagged as poor quality0
Sequence length290-300
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACGTTTTTCA20521.2540181135949742No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACCTTGTTCA13950.8525123140667588No Hit
CGGATGGTTNNGNACAACTTATNGNAAAGNTAAAGGAAACCCCAACATGC11530.7046212889741741No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA8760.5353410660376205No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA8720.532896586284024No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTNGGAACACGTTTTTCA8690.5310632264688268No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTNGGGTGGGAACACGTTTTTCA8200.5011183494872703No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNNGGTGGGAACACGTTTTTCA8060.4925626703496828No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNGGGTGGGAACACGTTTTTCA7900.4827847513352971No Hit
TGCTTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACGTTTTTCA7720.471784592444113No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATAGGGCTGGA7540.4607844335529291No Hit
TGCTTCTGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA7040.43022843663297355No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA6470.3953946001442243No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCAGGCCTGA6390.3905056406370314No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA5830.3562829240866813No Hit
CGGANGNTTNNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC5730.35017172470269015No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTNGGAACACCTTGTTCA5630.344060525318699No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNNGGTGGGAACACCTTGTTCA5610.34283828544190087No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTNGGGTGGGAACACCTTGTTCA5550.33917156581150615No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNGGGTGGGAACACCTTGTTCA5290.3232824474131293No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGTTTAA5270.3220602075363311No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATACGGATGAA5070.3098378087683489No Hit
TGCTTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACCTTGTTCA4770.2915042106163756No Hit
CGGANGNTTNNGNACAACTTATANAANAGGTAAANGAAACCCCAACATGC4630.282948531478788No Hit
CGGATGGTTNNGNACAACTTATNGNANAGNNAAAGGAAACCCCAACATGC4540.27744845203319607No Hit
CGGATGNTTNNGNACAACTTATANAANAGNTAAAGGAAACCCCAACATGC4430.2707261327108058No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTGGGAACACGTTTTTCA4380.2676705330188103No Hit
CGGNTGGTTNNGNACAACTTATNNNANAGNTAAAGGAAACCCCAACATGC4350.26583717320361294No Hit
CGGATGGTTNNGNACAACTTATNNNANAGNNAAAGGAAACCCCAACATGC4320.2640038133884156No Hit
TGCTTCNGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA4310.26339269345001654No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACGTTTTTCA4230.2585037339428236No Hit
TGCTTCTGANNGNTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA4100.25055917474363515No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCGAGCGTGA4090.24994805480523605No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACGTTTTTCA4080.24933693486683697No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNGGGTGGGAACACGTTTTTCA4030.2462813351748414No Hit
TGCTTCTGANNGNTCAAACACAGCNACCTNGGGTGGGAACACGTTTTTCA4010.24505909529804318No Hit
TGCTTCNGANNGCTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA3980.24322573548284585No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTNGGAACACGTTTTTCA3960.24200349560604767No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACGTTTTTCA3920.2395590158524512No Hit
CGGATGGTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC3910.23894789591405208No Hit
TGCTTCTGANNGNTCAAACACANCGACCTNGGGTGGGAACACGTTTTTCA3880.23711453609885474No Hit
TGCTNCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA3850.23528117628365744No Hit
TGCTNCNGANNGCTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA3820.2334478164684601No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACGTTTTTCA3770.23039221677646454No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTNGGGTNGGAACACGTTTTTCA3740.2285588569612672No Hit
TGCNTCTGANNGNTCAAACACANNNANCTCGGGTGGGAACACGTTTTTCA3630.22183653763887698No Hit
CGGATGGTTNNGNTGGTGTCGTNTNGTCCNGAGAGTCCAGTTTGTATCTG3610.2206142977620788No Hit
TGCTNCNGATNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA3540.216336458193285No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATAGGGCTGGA3530.2157253382548859No Hit
TGCTTCNGANNGNTCAAACACANNNANCTCGGGTNGGAACACGTTTTTCA3510.21450309837808768No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGAATGA3500.21389197843968855No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGTTTTA3400.20778077905569745No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCAGGCCAGA3360.20533629930210104No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATAGGGCTGGA3320.2028918195485046No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGCTTCA3280.20044733979490814No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATAGGGCTGGA3140.1918916606573206No Hit
CGGATGGTTNNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC3110.19005830084212327No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNGGGTGGGAACACCTTGTTCA3000.18333598151973304No Hit
TGCTTCNGANNGNTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA3000.18333598151973304No Hit
TGCTTCTGANNGNTCAAACACAGCNACCTNGGGTGGGAACACCTTGTTCA2980.18211374164293484No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATAGGGCTGGA2980.18211374164293484No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATAGGGCTGGA2960.1808915017661366No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATAGGGCTGGA2950.1802803818277375No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTGGGTTTCA2910.17783590207414107No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACCTTGTTCA2900.17722478213574197No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTGGGTATGA2890.17661366219734284No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATAGGGCTGGA2870.17539142232054464No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACCTTGTTCA2820.17233582262854907No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTNGGAACACCTTGTTCA2800.17111358275175087No Hit
CGGANGNTTNNGNACAACTTATANNANAGNTAAANGAAACCCCAACATGC2750.1680579830597553No Hit
TGCTTCNGANNGCTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA2750.1680579830597553No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTGGGAACACCTTGTTCA2730.16683574318295707No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGCTTTAA2720.16622462324455797No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACCTTGTTCA2710.16561350330615887No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTNGGGTNGGAACACCTTGTTCA2640.16133566373736508No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATCAGGCCTGA2640.16133566373736508No Hit
TGCTNCNGANNGCTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA2610.15950230392216777No Hit
CGGATGNTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC2520.15400222447657577No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATACGGATGAA2510.15339110453817667No Hit
CGGANGNTTNNGTACAACTTATANAANAGGTAAAGGAAACCCCAACATGC2470.1509466247845802No Hit
CGGNTGGTTNNGNACAACTTATNGNAAAGNTAAAGGAAACCCCAACATGC2460.1503355048461811No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGGTTTAA2440.1491132649693829No Hit
CGGANGNTTNNGNACAACTTATANNANAGNTAAAGGAAACCCCAACATGC2430.14850214503098377No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATTTGGTTTAA2430.14850214503098377No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCAGGTAAAA2420.14789102509258467No Hit
TGCTTCNGANNGNTCAAACACANNNANCTCGGGTNGGAACACCTTGTTCA2400.14666878521578647No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACCTTGTTCA2380.14544654533898824No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATCAGGCCTGA2370.1448354254005891No Hit
CGGATGGTTNNGNACAACTTATNGNAAAGNNAAAGGAAACCCCAACATGC2370.1448354254005891No Hit
TGCNTCTGANNGNTCAAACACANNNANCTCGGGTGGGAACACCTTGTTCA2370.1448354254005891No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATCAGGCCTGA2350.1436131855237909No Hit
TGCTNCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA2330.14239094564699267No Hit
CGGATGGTTNNGNACAACTTATANNANAGNTAAANGAAACCCCAACATGC2310.14116870577019447No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATACGGATGAA2290.13994646589339624No Hit
CGGATGGTTNNGNACAACTTATNGNANAGNTAAAGGAAACCCCAACATGC2270.13872422601659803No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATCAGGCCTGA2240.13689086620140067No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATCAGGCCTGA2230.13627974626300157No Hit
TGCTTCTGANNGNTCAAACACANCGACCTNGGGTGGGAACACCTTGTTCA2220.13566862632460247No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGATGGA2200.13444638644780424No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATACGGATGAA2200.13444638644780424No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATTTGGTTTAA2150.1313907867558087No Hit
TGCTNCNGATNGNTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA2140.1307796668174096No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATCGAGCGTGA2130.13016854687901047No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATCAGGCCTGA2130.13016854687901047No Hit
CGGATGNTTNNGNACAACTTATNNNANAGGTAAANGAAACCCCAACATGC2120.12955742694061137No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATACGGATGAA2110.12894630700221224No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATTTGGTTTAA2100.12833518706381317No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATTTGGTTTAA2100.12833518706381317No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATAGGACTTGA2090.12772406712541404No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATACGGATGAA2080.12711294718701494No Hit
CGGNTGGTTNNGNACAACTTATNNNANAGNNAAAGGAAACCCCAACATGC2060.1258907073102167No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATCAGGCCTGA2060.1258907073102167No Hit
CGGATGGTTNNGNACAACTTATNGCAAAGNTAAAGGAAACCCCAACATGC2020.12344622755662027No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATACGGATGAA2020.12344622755662027No Hit
CGGNTGGTTNNGNACAACTTATNNNANAGGTAAAGGAAACCCCAACATGC2010.12283510761822115No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGGTTGA1990.12161286774142292No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATTTGGTTTAA1980.12100174780302383No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATGGGGTTTGA1930.11794614811102826No Hit
GTCTCTCAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATAGGGCTGGA1930.11794614811102826No Hit
CGGANGNTTTNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC1920.11733502817262917No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTGAGTTCCA1910.11672390823423005No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATACGGATGAA1900.11611278829583094No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATTTGGTTTAA1860.1136683085422345No Hit
CGGNTGGTTNNGNACAACTTATNGNAAAGNNAAAGGAAACCCCAACATGC1840.11244606866543629No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATCGAGCGTGA1830.11183494872703717No Hit
CGGATGGTTNNGNACAACTTATCGNAAAGNTAAAGGAAACCCCAACATGC1820.11122382878863805No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATCGAGCGTGA1810.11061270885023894No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGCTAAAA1810.11061270885023894No Hit
GTCNCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATAGGGCTGGA1780.10877934903504162No Hit
GTCTCTCAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATCAGGCCTGA1770.1081682290966425No Hit
CGGATGNTTNNGTACAACTTATANNANAGGTAAAGGAAACCCCAACATGC1770.1081682290966425No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGAAAGA1740.10633486928144519No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATCGAGCGTGA1730.10572374934304607No Hit
GTCTCTCAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGGTTTAA1720.10511262940464695No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGTTGCA1720.10511262940464695No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGGTTTTA1720.10511262940464695No Hit
GTCNCTCAGNNGNTACACGGCANGNTCAGNNTTCTGGATATAGGGCTGGA1690.10327926958944962No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATTTGGTTTTA1660.1014459097742523No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATTTGGAATGA1650.10083478983585319No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTGGAACTCA1650.10083478983585319No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGGAATGA1640.10022366989745407No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)