FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004953582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54744
Sequences flagged as poor quality0
Sequence length35-300
%GC49

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN855615.629110039456378No Hit
GGGAGCGCCCGAATCTGGCTTGGCGGAATATCTCTTTGACAAGCACACCC45078.232865702177408No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG17483.193043986555604No Hit
GGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACGA11452.091553412246091No Hit
GGGGAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAA6881.2567587315504896No Hit
GGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG6391.1672512056115738No Hit
GGGGATGCTTGCATGTGTAATCTTACTAAGAGCTAATAGAAAGGCTAGGACCAAACCATCCG4930.9005553119976618No Hit
GGGGAGGAAACCCTCAGTCCATATAAGCGACACGGCTGAGTACTTCTGTG4280.781820838813386No Hit
GGGGGTTTTTCTGCTGTGGGTACGTGAGCAGGAAACATGGAGAAGAATCC4020.7343270495396755No Hit
GGGATCTGCAGTGCTAGAAGTGGGGCCGAGAGGGAGACCCAGTACTTCGG3970.7251936285255005No Hit
GGGGAAAAAAGGATAAACATCTGTCTCTGCGCATTGCAGACACCCAGACT3630.66308636562911No Hit
GGGGAGGAAACCCTCTGTGCAGTGGAGTGACACAGCTGAGTACTTCTGTG3210.5863656291100394No Hit
GGGACAACTGGGGGACTCAGCAATGTACTTCTGTGCAATGAGAGAGGGCT3060.5589653660675142No Hit
GGGGAGAACAGCGTCTTTTACCTGTCACCGAAGCCAGGAAGCCCCGTTTG3030.5534853134590092No Hit
GGGAAGAAGAAGCATGGAAGATTAATTGCCACAATAAACATACAGGAAAA3020.5516586292561741No Hit
GGGACTGAGAAGGCTCACGAAGGGCATCCGCAATGTTGGTTTCACTGAGA2710.49503141896828873No Hit
GGGGAGAAGTAACTCTTATAACTGGAGGTTGCAGGTCAATGACTGATCTT2690.49137805056261874No Hit
GGGGTGACTTCCCTGGTCACCAAGGCAGTGCATGCATGTTGGGGTTTCCT2680.4895513663597837No Hit
GGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTG2480.4530176823030835No Hit
GGGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACG2460.44936431389741344No Hit
GGGGGCTTTCTGATGGGAGGGAACTCTGAAGGTAGAAAGACTATTGTTAC2320.4237907350577232No Hit
GGGGGGCAAAGGGACTCATCTAATCATCCAGCCCTATATCCAGAAGCCTG2320.4237907350577232No Hit
GGGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGTCTGGGA2310.4219640508548882No Hit
GGGGAGAATGTGGAGCAGCATCCTTCAACCCTGAGTGTCCAGGAGGGAGA2280.41648399824638316No Hit
GGGGGACTCAGCAATGTACTTCTGTGCAATGAGAGAGGGCTTAAGGAGCG2070.3781236299868479No Hit
GGGGGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGC1960.35803010375566274No Hit
GGGGGAGTTCTTTCCAGACAAAGCTTATCTTCGGACAGGGAACGGAGTTA1920.35072336694432266No Hit
GGGGACAGAAGGAATGGAGACCCTCTTGGGCCTGCTTATCCTTTGGCTGC1900.34706999853865267No Hit
GGGGGTTCATATGTAAAATGAAGGGTCTGTGGAAGGACATGAATAAAGCA1870.3415899459301476No Hit
GGGGAGTTTTCAGTGAACTGGACAGAAAAAAAAAATGAAGAAGCTACTAG1840.33610989332164254No Hit
GGGAGCCTTACAAAGACATCCAGCTCTAAGGAGCTCAAAACATCCTGAGG1800.3288031565103025No Hit
GGGGTCACTGACACAAAGGAAAAAATGCAAAACAGGTAGTCTTAAATAAG1700.31053631448195235No Hit
GGGGAAGGTTTTTCGTAATGATGCCTGTGGTAGTGCTTTGACAGCACAAC1690.30870963027911735No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAACCAAACCATCCG1630.2977495250621073No Hit
GGGAAAGCTGCTGACAGCCGTGAGAAGAAAAGCAGCGGAGACAAGCTGAC1550.2831360514394271No Hit
GGGATACAAAGCCCCCGCACCAATAGGATCCTCCCGAATCAACCCTGACC1530.2794826830337571No Hit
GGGATTTCTTCTCTCACCATGCCAGGTTCACTTCACAGTACAGAGTCCTG1480.27034926201958204No Hit
GGGGATTTTTGGTGATGGGACTCGACTCTCCATCCTAGAGGACCTGAACA1470.26852257781674704No Hit
GGGGAGGCCAGTGTTGTGGGCTTCCTGCTGGGACTGAGAAGGCTCACGAA1340.24477568317989185No Hit
GGGAGGCAACCCATTTAGGAGAAGTTGGATGAAGAGGGAGAGGGAGATGC1230.22468215694870672No Hit
GGGAAGCAGAAAGCTAAGAAGCCACCGGAGCTCATGTTTGTCTACAGCTA1230.22468215694870672No Hit
GGGATACCAGTTATTCAGAGAAATAAAATGGGCTTTAACTAAATGGAATC1210.22102878854303667No Hit
GGGGGTATTCAGGAGGAGGTGCTGACGGACTCACCTTTGGCAAAGGGACT1210.22102878854303667No Hit
GGGGGAGTCTGCTGCCTCCTCCCAGACATCTGTATATTTCTGCGCCAGCA1190.21737542013736663No Hit
GGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACAGTGC1150.2100686833260266No Hit
GGGGTCAGAGCTGCTCCTATGCTGTGAGTGGTCTGATTTTCTCAGCATCT1150.2100686833260266No Hit
GGGAGAGATGTCTGTGCAGGAGGCAGAGACCGTGACCCTGAGCTGCACAT1140.20824199912319158No Hit
GGGGACAATGAGCAGTTCTTCGGGCCAGGGACACGGCTCACCGTGCTAGA1130.20641531492035659No Hit
GGGGGTGTTTATCTTGTGAGCCATTCTCCATATTTCAGATATAAGATTTC1120.20458863071752156No Hit
AGGATGCTCAAACCTGCTTCTTTCTCCCAGGTGATTTTCATAGTTCCTCATTTCCTCATACCAAACCATCCG1110.20276194651468654No Hit
GGGGATACAGAAGTGGCGCCTCTGAGAAAAGAAGGTTGGAATTATCGTAA1110.20276194651468654No Hit
GGGAGGAAGAAAAGGAAAGTGAAGAAGCCATCCTAGCAGATGATGATGAA1090.19910857810901653No Hit
GGGGGGACTGACTAAGAAACACTGTGGGATGGATAGCAGCTATAAATTGA1090.19910857810901653No Hit
GGGATATTCCGAAATCCTCCAACAGAGACCTGTGTGAGCTTCTGCTGCAG1090.19910857810901653No Hit
GGGATCTTTGCGCCAGCAGCCCCGACAGGGTGGGCACCGGGGAGCTGTTT1070.1954552097033465No Hit
GGGATCGTTGCCTTAAGGGAGCTGGGGGGTTGTCAACGGGCCAGAGGTGG1060.19362852550051146No Hit
CGGATTGATTCTATTCAACTCCCTTACTCTCCATTACATTCTATTCCATT1030.18814847289200645No Hit
GGGAACTTAAACTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAA1030.18814847289200645No Hit
GGGGTCTGTGCTAAGGGAGGTGGGGAAGGAGAAGGAATTCTGGGCAGCCC1030.18814847289200645No Hit
GGGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCT1010.18449510448633638No Hit
GGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAACCATCCG990.18084173608066637No Hit
GGGGGGGAGTTACCAACTCACTTTCGGGAAGGGGACCAAACTCTCGGTCA970.17718836767499635No Hit
GGGTGCACCCATCACCCGAGCAGTATTCACTGCACCATATTTATAGTCTT960.17536168347216133No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT930.1698816308636563No Hit
GGGGGGGAACAACAGACTCGCTTTTGGGAAGGGGAACCAAGTGGTGGTCA930.1698816308636563No Hit
GGGACACGTGGGGAGTGGGGTGGGGGGAGCTATTTCCAAGGCCCCTCCCTGTTTCCCCCAAG920.16805494666082127No Hit
GGGACAACAATTATCAATATAAAACCCCCTGCCATAACCCAATACCAAACCATCCG910.16622826245798625No Hit
GGGCACCTTACTACCAGACAACCTTAGCCAAACCATCCG910.16622826245798625No Hit
GGGTAGCCCAGAGAAGTCTGTTCCAGGACGGGCTCTTTCAGGAGCAGCTA890.16257489405231623No Hit
GGGGATGAAGTGTCTACCTTCTGCAGACTACAGTGGCTCAGGAACCGGGG880.16074820984948124No Hit
GGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACAT880.16074820984948124No Hit
GGGGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCC850.15526815724097617No Hit
GGGGTTTCCTTTACCTTTTCTATAAGTTGTACCAAACCATCCG850.15526815724097617No Hit
GGGAACACAGTGTGATTTATAACCAGGGAGGAAAGCTTATCTTCGGACAG830.15161478883530616No Hit
GGGCGAGCAGTTTTGTTGATTTAACTGTTGAGGTTTAGGGCTAAGCATAG830.15161478883530616No Hit
GGGGGAGGAAACCCTCTGTGCAGTGGAGTGACACAGCTGAGTACTTCTGT820.14978810463247114No Hit
GGGGATTTTGGCCATGGCTTTGCAGAGCACTCTGGGGGCGGTGTGGCTAG810.14796142042963611No Hit
GGGACACTAAAGATGAACTATTCTCCAGGCTTAGTATCTCTGATACTCTT800.14613473622680112No Hit
GGGGAGGAAGAAAAGGAAAGTGAAGAAGCCATCCTAGCAGATGATGATGA790.1443080520239661No Hit
GGGACATATTTCCTCTTCTTCTACCTTTAGCTTTTATTCCACAGCTCTTG780.1424813678211311No Hit
GGGATAAAACCCCCTGCCATAACCCAATACCAAACCATCCG780.1424813678211311No Hit
GGGATAACCATGCACACTACTATAACCACCCTAACCCTGACTTCCCTAAT770.14065468361829606No Hit
GGGAAGAGGGGTAGGGAAGGAAGGAGAGGTCACTCAGATTCCTTCTCACA760.13882799941546106No Hit
GGGAGAGCGGGCTGAATGCAATGGAGTGTGCATTACATTTGGAAAAAAAT750.13700131521262604No Hit
GGGAGTATAGGCTCCCTAGACCTGCTCCCCAGCTGTGGTGTAAAAATGGA750.13700131521262604No Hit
GGGGGTAAAGCAGATTCTTTTTATGATTTTTAAAGTAGAAATATCCATTC740.13517463100979102No Hit
GGGATAACTGGAGGTTGCAGGTCAATGACTGATCTTAATTGGGAAGAACA740.13517463100979102No Hit
GGGAGTGCTTTGCCAACCTGGCCTGTTTGATCTGGTTTTTGTTGTTGAGC740.13517463100979102No Hit
GGGGATTCTCTGCCACAGCCCTGGACTGCTAGGAGGGCAGATCATATGTC730.13334794680695602No Hit
GGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCA720.131521262604121No Hit
GGGATCTAATGCCTGATGACCTGTCACTGCCTCCCATCACCCCCAGATGG720.131521262604121No Hit
GGGGAAAAGGATGTGTGGGCTTCTGGGTGTTTTTGACTGACTAAGAAACA720.131521262604121No Hit
GGGACCTTACTACCAGACAACCTTAGCCAAACCATCCG700.127867894198451No Hit
GGGGTTTTTGCAATGACTTAGAACACTGTGTATCTAACTTTGGAAATGAG700.127867894198451No Hit
GGGGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATC690.12604120999561594No Hit
GGGCGAAAAGAGAAGAGGAATTTCCCCCTGATCCTGGAGTCGCCCAGCCC690.12604120999561594No Hit
GGGAGAGTTATCAAGAGACACTCCCCCCTCCATTCCCACCCCTCATACCAAACCATCCG690.12604120999561594No Hit
GGGGGCTGATGATGTCACTGACACAAAGGAAAAAATGCAAAACAGTTGCA690.12604120999561594No Hit
GGGGAGAGTGTTTATCTTGTGAGCCATTCTCCATATTTCAGATATAAGAT680.12421452579278094No Hit
GGGATTGCAGGAAGGAGATTTGCAAGAAACGGGGCAGGGGCTTGCAGTAG660.12056115738711091No Hit
ATGCCTCCATGCCATCGTACGTTCCCTGATAGAACCACTGGGCTAAGCAA660.12056115738711091No Hit
CAGCCTCTCATCCATTCTTCCTGCCCATCGACCCACCCATTCTCCCATCC660.12056115738711091No Hit
GGGAGCAAATCTGTGCACCCATCTCTCTCTTCCTCATCTCCTTTCCTTAT650.11873447318427591No Hit
GGGGAGGACTCCTGGTTCTGGGTGCTGGGAGAGCGATGGGGCTCTCAGCG640.11690778898144089No Hit
AGGGCTCCCACACTCTCAGCCACTATACACCGGTGCCCTAATTACCCTAAGGCCAAGTACCAAACCATCCG640.11690778898144089No Hit
GGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTAT630.11508110477860588No Hit
GGGGGCACTGCATCAAGTGTACTGTGCTGGAAAGGTCCGCCATAGGAAAT630.11508110477860588No Hit
GGGACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATC620.11325442057577087No Hit
GGGGAGATATAAGATTTCAGTTCTCAGTGAGTCTAAGTGACAGAAGGAAT620.11325442057577087No Hit
GGGATAAACAACATAAGCTTCTGACTCTTACCTCCCTCTCTCCTACTCCT620.11325442057577087No Hit
GGGCAAAGCCCCCGCACCAATAGGATCCTCCCGAATCAACCCTGACCCCT610.11142773637293583No Hit
GGGGGTTTAAGGTTTGAATCCTCAGTGAACCAGGGCAGAAAAGAATGATG610.11142773637293583No Hit
GGGAACACACCTCAACCACTTTCCTCCCAATACAGTGTGCTCTGGCCCAG610.11142773637293583No Hit
GGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAAT600.10960105217010083No Hit
GGGACTGAACTTTGAGCTTCAGGCAGCACAACTCACATTTGTGCAAAGAG600.10960105217010083No Hit
GGGATGAACCATAACCAATACTACCAATCAATACTCATCATTAATAATCA580.1059476837644308No Hit
GCCCAGAGGGCAAGTAGCCTCCTTCCTCATACCAAACCATCCG580.1059476837644308No Hit
GGGGCAAGCAGCTATTGCAGAAGTCTTAGATTAGAGAGAACAGTAGAGGA580.1059476837644308No Hit
GGGAAAAACAAAAGTGCCAAGCACCTCTCTCTGCACATTGTGCCCTCCCA580.1059476837644308No Hit
GGGGCTACTCCCCCTTACTACTGCCCACGAAACTCTCACCACCCTACTGC580.1059476837644308No Hit
GGGTGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTT580.1059476837644308No Hit
GGGGCAATAATCCCCATCCTCCATATATCCAAACAACAAAGCATAATATT570.10412099956159579No Hit
GGGATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGG570.10412099956159579No Hit
GGGATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA570.10412099956159579No Hit
GGGAGACCTGGTGCTGACAGTGCTCATTGCCCTGGCCGTGTACTTCCTGG570.10412099956159579No Hit
GGGAACGAATTGCTGTTACATTGAACAAGACAGCCAAACATTTCTCCCTG570.10412099956159579No Hit
GGGGAAATTCTTTAAACCTAGAATCAGACACAAAAACTGAACTCTGGGTC560.10229431535876078No Hit
GGGGAGACAGATGCTTCATTCCTGTATGGGGTGGTATTCCTGCCATGGGT550.10046763115592577No Hit
GGGAGATATAAGATTTCAGTTCTCAGTGAGTCTAAGTGACAGAAGGAATG550.10046763115592577No Hit
GGGGAAAAGGCCATATCTCTAGGTCTTAGGTATGAAAAGGATGTGTGGGC550.10046763115592577No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)