FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004953583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54744
Sequences flagged as poor quality0
Sequence length290-300
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGATGGTTNNGNACAACTTATNGNAAAGNTAAAGGAAACCCCAACATGC5310.9699693117053925No Hit
CTTGGGGGANNCNCTCTGCGTTNANACCANTGCTTCCGCGGACAGGCATA5170.9443957328657021No Hit
CGGATGGTTNNGNTAAGGTTGTNTNGTAGNAAGGTGGAGTGGGTTTGGGG3000.5480052608505042No Hit
CGGANGNTTNNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC2740.5005114715767938No Hit
CGGANGNTTNNGNACAACTTATANAANAGGTAAANGAAACCCCAACATGC2460.44936431389741344No Hit
CGGATGNTTNNGNACAACTTATANAANAGNTAAAGGAAACCCCAACATGC2400.4384042086804033No Hit
CGGATGGTTNNGNACAACTTATNNNANAGNNAAAGGAAACCCCAACATGC2270.41465731404354816No Hit
CTTGNGNGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCATA2260.41283062984071317No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACGTTTTTCA2240.4091772614350431No Hit
CGGATGGTTNNGNACAACTTATNGNANAGNNAAAGGAAACCCCAACATGC2150.392737103609528No Hit
CGGNTGGTTNNGNACAACTTATNNNANAGNTAAAGGAAACCCCAACATGC2140.390910419406693No Hit
CTTGGGGGANNCNCTCTGCGTTNANANCANNGCTTCCGCGGACAGGCATA2030.3708168931755078No Hit
CTTGNGNGANNCNCTCTGCGTTGNTANCACTGCTNCCGCGGACAGGCATA2010.36716352476983777No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACCTTGTTCA1950.3562034195528277No Hit
CTTGGGGGANNCNCTCTGCGTTNNNANCANNGCTTCCGCGGACAGGCATA1870.3415899459301476No Hit
CGGATGGTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC1770.32332310390179747No Hit
CTTNGGGGANNCNCTCTGCGTTNNNANCANTGCTTCCGCGGACAGGCATA1740.31784305129329243No Hit
CGGATGGTTNNGNTGGTGTCGTNTNGTCCNGAGAGTCCAGTTTGTATCTG1650.30140289346777727No Hit
CGGATGGTTNNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC1590.2904427882507672No Hit
CGGATGNTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC1470.26852257781674704No Hit
CTTGGGNGANNCNCTCTGCGTTGNTANCANTGCTTCCGCGGACAGGCATA1460.26669589361391205No Hit
CGGANGNTTNNGNTAAGGTTGTCNGGNAGTAAGGTGGAGTGGGTTTGGGG1400.255735788396902No Hit
CTTGGGGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCATA1380.2520824199912319No Hit
CGGANGNTTNNGNACAACTTATANNANAGNTAAAGGAAACCCCAACATGC1340.24477568317989185No Hit
CGGATGGTTNNGNTAAGGTTGTNTNGNAGNNAGGTGGAGTGGGTTTGGGG1310.2392956305713868No Hit
CGGNTGGTTNNGNACAACTTATNGNAAAGNTAAAGGAAACCCCAACATGC1280.23381557796288177No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA1250.22833552535437676No Hit
CGGNTGGTTNNGNTAAGGTTGTNNNGNAGNAAGGTGGAGTGGGTTTGGGG1220.22285547274587167No Hit
CGGANGNTTNNGNTAAGGTTGTCNGGNAGTAAGGNGGAGTGGGTTTGGGG1210.22102878854303667No Hit
CGGANGNTTNNGNACAACTTATANNANAGNTAAANGAAACCCCAACATGC1190.21737542013736663No Hit
CGGATGGTTNNGNACAACTTATANNANAGNTAAANGAAACCCCAACATGC1190.21737542013736663No Hit
CTTGGGGGANNCNCTCTGCGTTNANACCANNGCTTCCGCGGACAGGCATA1180.21554873593453164No Hit
CTTGGGGGANNCNCTCTGCGTTNATACCANTGCTTCCGCGGACAGGCATA1170.21372205173169662No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTNGGAACACGTTTTTCA1130.20641531492035659No Hit
CGGATGGTTNNGNACAACTTATNGNAAAGNNAAAGGAAACCCCAACATGC1130.20641531492035659No Hit
CGGATGGTTNNGNACAACTTATNGNANAGNTAAAGGAAACCCCAACATGC1120.20458863071752156No Hit
CTTGGGGGANNCNCTCTGCGTTGANACCANTGCTTCCGCGGACAGGCATA1100.20093526231185155No Hit
CGGANGNTTNNGTACAACTTATANAANAGGTAAAGGAAACCCCAACATGC1090.19910857810901653No Hit
CTTNGGGGANNCNCTCTGCGTTNANACCANTGCTTCCGCGGACAGGCATA1080.1972818939061815No Hit
CGGATGNTTNNGNACAACTTATNNNANAGGTAAANGAAACCCCAACATGC1060.19362852550051146No Hit
CTTGNGNGANNCNCTCTGCGTTGNNANCANTGCTTCCGCGGACAGGCATA1020.1863217886891714No Hit
CTTGNGNGANNCNCTCTGCGTTGNNANCANTGCTNCCGCGGACAGGCATA1020.1863217886891714No Hit
CGGATGGTTNNGNTAAGGTTGTNNNGNAGNNAGGTGGAGTGGGTTTGGGG1010.18449510448633638No Hit
CTTGNGNGANNCCCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCATA1010.18449510448633638No Hit
CTTGGGNGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCATA990.18084173608066637No Hit
CGGATGGTTNNGNACAACTTATCGNAAAGNTAAAGGAAACCCCAACATGC990.18084173608066637No Hit
CTTGGGGGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCATA970.17718836767499635No Hit
CGGATGNTTNNGNTAAGGTTGTCNGGNAGNAAGGTGGAGTGGGTTTGGGG970.17718836767499635No Hit
CGGATGGTTNNGNTAAGGTTGTCNNGNAGTAAGGTGGAGTGGGTTTGGGG970.17718836767499635No Hit
CGGANGNTTTNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC970.17718836767499635No Hit
CGGNTGGTTNNGNACAACTTATNNNANAGNNAAAGGAAACCCCAACATGC950.17353499926932633No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNNGGTGGGAACACGTTTTTCA950.17353499926932633No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA920.16805494666082127No Hit
CTTNGGGGANNCNCTCTGCGTTNNNANCANNGCTTCCGCGGACAGGCATA910.16622826245798625No Hit
CGGATGNTTNNGTACAACTTATANNANAGGTAAAGGAAACCCCAACATGC910.16622826245798625No Hit
CTTGGGGGANNCNCTCTGCGTTNANANCANTGCTTCCGCGGACAGGCATA890.16257489405231623No Hit
TGCTTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACGTTTTTCA890.16257489405231623No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTNGGGTGGGAACACGTTTTTCA880.16074820984948124No Hit
CTTNGGGGANNCNCTCTGCGTTNANACCANNGCTTCCGCGGACAGGCATA870.15892152564664622No Hit
CGGNTGGTTNNGNACAACTTATNGNAAAGNNAAAGGAAACCCCAACATGC870.15892152564664622No Hit
CGGATGGTTNNGNACAACTTATNGCAAAGNTAAAGGAAACCCCAACATGC860.1570948414438112No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNGGGTGGGAACACGTTTTTCA850.15526815724097617No Hit
CGGATGGTTNNGNCCTAGCCTTNCNATTANCTCTTAGTAAGATTACACAT840.15344147303814115No Hit
TGCTTCTGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA840.15344147303814115No Hit
CTTGGGGGANNCNCTCTGCGTTGNNANCANTGCTNCCGCGGACAGGCATA830.15161478883530616No Hit
CGGNTGGTTNNGNTAAGGTTGTNTNGTAGNAAGGTGGAGTGGGTTTGGGG830.15161478883530616No Hit
CTTGGGNGANNCNCTCTGCGTTNNNANCACTGCTNCCGCGGACAGGCATA810.14796142042963611No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTNGGAACACCTTGTTCA790.1443080520239661No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA770.14065468361829606No Hit
CGGANGNTTNNGNTGGTGTCGTTNTGNCCTGAGAGTCCAGTTTGTATCTG770.14065468361829606No Hit
CGGANGNTTNNGNTAAGGTTGTCNNGNAGNAAGGNGGAGTGGGTTTGGGG760.13882799941546106No Hit
CGGNTGGTTNNGNACAACTTATNNNANAGGTAAAGGAAACCCCAACATGC760.13882799941546106No Hit
CGGATGGTTNNGNTAAGGTTGTCNGGNAGTAAGGTGGAGTGGGTTTGGGG750.13700131521262604No Hit
CGGANGNTTNNGNTGGTGTCGTTNTGNCCTGAGANTCCAGTTTGTATCTG740.13517463100979102No Hit
CGGANGGTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC710.12969457840128598No Hit
CGGATGGTTNNGNTGGTGTCGTNTNGNCCNNAGAGTCCAGTTTGTATCTG690.12604120999561594No Hit
CGGATGGTTNNGNTAAGGTTGTNTNGNAGNAAGGTGGAGTGGGTTTGGGG690.12604120999561594No Hit
CTTGGTGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCATA680.12421452579278094No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNGGGTGGGAACACCTTGTTCA680.12421452579278094No Hit
CTTGNGNGACNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCATA680.12421452579278094No Hit
CTTNGGGGANNCNCTCTGCGTTNNNANCACTGCTTCCGCGGACAGGCATA660.12056115738711091No Hit
CGGATGNTTNNGNTGGTGTCGTTNTGNCCNGAGAGTCCAGTTTGTATCTG660.12056115738711091No Hit
CTTGGTGGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCATA660.12056115738711091No Hit
CGGATGNTTNNGNTAAGGTTGTCNNGNAGTAAGGTGGAGTGGGTTTGGGG650.11873447318427591No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTGGGAACACGTTTTTCA650.11873447318427591No Hit
TGCTTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACCTTGTTCA640.11690778898144089No Hit
CGGNTGGTTNNGNTAAGGTTGTNTNGTAGNNAGGTGGAGTGGGTTTGGGG630.11508110477860588No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTNGGGTNGGAACACGTTTTTCA610.11142773637293583No Hit
CGGATGGTTNNGNTGGTGTCGTNNNGNCCNNAGAGTCCAGTTTGTATCTG610.11142773637293583No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACGTTTTTCA610.11142773637293583No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGAATGA600.10960105217010083No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNCTTCTGGATATTTGGTATGA600.10960105217010083No Hit
CGGANGNTTNNGNTAAGGTTGTCNNGNAGNAAGGTGGAGTGGGTTTGGGG600.10960105217010083No Hit
CGGATGGTTNNGNTAAGGTTGTNTNGTAGNNAGGTGGAGTGGGTTTGGGG600.10960105217010083No Hit
CGGNTGGTTNNGNTAAGGTTGTNNNGNAGNNAGGTGGAGTGGGTTTGGGG590.10777436796726582No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTNGGGTGGGAACACCTTGTTCA590.10777436796726582No Hit
CGGATGGTTNNGNTAAGGTTGTCNNGNAGNAAGGNGGAGTGGGTTTGGGG580.1059476837644308No Hit
TGCTTCNGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA570.10412099956159579No Hit
CGGNTGGTTNNGNTGGTGTCGTNNNGNCCNGAGAGTCCAGTTTGTATCTG550.10046763115592577No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)