FastQCFastQC Report
Wed 8 May 2024
EGAF00004953586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59743
Total Bases12.3 Mbp
Sequences flagged as poor quality0
Sequence length35-300
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT16522.765177510335939No Hit
GGGAGCGCCCGAATCTGGCTTGGCGGAATATCTCTTTGACAAGCACACCC13512.261352794469645No Hit
GGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTT6321.0578645196926837No Hit
GGGGGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGC5270.8821117118323486No Hit
GGGAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCT3630.6076025643171585No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3410.570778166479755No Hit
GGGGAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAA3210.5373014411730245No Hit
GGGAGGAGGAAGCTACATACCTACATTTGGAAGAGGAACCAGCCTTATTG3090.5172154059889861No Hit
GGGATAACCAGGGAGGAAAGCTTATCTTCGGACAGGGAACGGAGTTATCT2840.475369499355573No Hit
GGGAGACTGGATGTGTTTTTGACAGGATATGTAACACAGTGTGATTTATA2800.468674154294227No Hit
GGGAGATCAGAAGAGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGCA2680.44858811911018864No Hit
GGGACAAAGACATCCAGCTCTAAGGAGCTCAAAACATCCTGAGGACAGTG2630.440218937783506No Hit
GGGGAGGAGGAAGCTACATACCTACATTTGGAAGAGGAACCAGCCTTATT2570.43017592019148687No Hit
GGGAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTTCC2470.41343755753812156No Hit
GGGGAGATATAAGATTTCAGTTCTCAGTGAGTCTAAGTGACAGAAGGAAT2440.40841604874211207No Hit
GGGGAGACTGGATGTGTTTTTGACAGGATATGTAACACAGTGTGATTTAT2410.40339453994610247No Hit
GGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACGA2250.37661315970071807No Hit
GGGATTTTGGCCATGGCTTTGCAGAGCACTCTGGGGGCGGTGTGGCTAGG2240.37493932343538156No Hit
GGGGATGAAAAGGATGTGTGGGCTTCTGGGTGTTTTTGACTGACTAAGAA2130.3565271245166798No Hit
GGGAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAA2070.34648410692466064No Hit
GGGAGAAACACAGTGGAAGATTAAGAGTCACGCTTGACACTTCCAAGAAA2020.338114925597978No Hit
GGGAACACAGTGTGATTTATAACCAGGGAGGAAAGCTTATCTTCGGACAG2020.338114925597978No Hit
GGGGGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCAT1960.3280719080059588No Hit
GGGGACACTAAAGATGAACTATTCTCCAGGCTTAGTATCTCTGATACTCT1940.32472423547528584No Hit
GGGAGGGAGACAGCGCTGTTATCAAGTGTACTTATTCAGACAGTGCCTCA1920.32137656294461275No Hit
GGGGAGAATGTGGAGCAGCATCCTTCAACCCTGAGTGTCCAGGAGGGAGA1890.3163550541486032No Hit
GGGAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCT1890.3163550541486032No Hit
GGGAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTC1880.3146812178832667No Hit
GGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTA1870.3130073816179301No Hit
GGGAGAGCGGGCTGAATGCAATGGAGTGTGCATTACATTTGGAAAAAAAT1850.3096597090872571No Hit
GGGGAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGC1810.302964364025911No Hit
GGGACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGC1790.29961669149523795No Hit
GGGGAGTGTGATTTATAACCAGGGAGGAAAGCTTATCTTCGGACAGGGAA1760.29459518269922835No Hit
GGGGAACACAGTGTGATTTATAACCAGGGAGGAAAGCTTATCTTCGGACA1760.29459518269922835No Hit
GGGGATTTTGGCCATGGCTTTGCAGAGCACTCTGGGGGCGGTGTGGCTAG1750.29292134643389184No Hit
GGGAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGT1740.2912475101685553No Hit
GGGGGGCACTGCCAGTAAACTCACCTTTGGGACTGGAACAAGACTTCAGG1720.28789983763788224No Hit
GGGGAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTT1690.2828783288418727No Hit
GGGGACTGACTAAGAAACACTGTGGGATGGATAGCAGCTATAAATTGATC1670.2795306563111996No Hit
GGGGGATGATAGAAGTAACTCTTATAACTGGAGGTTGCAGGTCAATGACT1620.27116147498451704No Hit
GGGGAGATCAGAAGAGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGC1610.26948763871918047No Hit
GGGATTGCAGACACCCAGACTGGGGACTCAGCTATCTACTTCTGTGCAGA1600.26781380245384395No Hit
GGGGGATCTTAATTGGGAAGAACAAGGATGACATCCATTCGAGCTGTATT1590.26613996618850744No Hit
GGGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGT1530.2560969485964883No Hit
GGGAGGATATGTAACACAGTGTGATTTATAACCAGGGAGGAAAGCTTATC1500.2510754398004787No Hit
GGGAGAAGAGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGCATGGCA1440.24103242220845955No Hit
GGGAGCATTTTGGTGATGGGACTCGACTCTCCATCCTAGAGGACCTGAAC1370.2293155683511039No Hit
GGGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCT1360.22764173208576735No Hit
GGGGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCC1350.22596789582043086No Hit
GGGAGTTGCTGCTGGGCTCATTGCAGCTCAGACACAGCAAAAGAGCCTAG1350.22596789582043086No Hit
GGGATATCTCTAGGTCTTAGGTATGAAAAGGATGTGTGGGCTTCTGGGTG1320.22094638702442126No Hit
GGGAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCT1270.21257720569773866No Hit
GGGAGTACTTCGGCGCCGGGACCCGGCTCTCAGTGCTGGAGGACCTGAAA1260.21090336943240212No Hit
GGGGATATCTCTAGGTCTTAGGTATGAAAAGGATGTGTGGGCTTCTGGGT1200.20086035184038298No Hit
GGGGGCAGACCAGAATCCTGCCCTGGGCCTTGCCTGGTCTGCCTCACTCT1190.19918651557504644No Hit
GGGGGACTGACTAAGAAACACTGTGGGATGGATAGCAGCTATAAATTGAT1190.19918651557504644No Hit
GGGGAGAAGAGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGCATGGC1180.1975126793097099No Hit
GGGGGAGGAAAGCTTATCTTCGGACAGGGAACGGAGTTATCTGTGAAACC1170.1958388430443734No Hit
GGGATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTTCCCACC1160.19416500677903686No Hit
GGGGATTGCAGCTCAGACACAGCAAAAGAGCCTAGAACCTGGGTCCTAGT1110.18579582545235424No Hit
GGGAGTCTTTCTTCTGGTACAGACAGGATTGCAGGAAAGAACCTAAGTTG1110.18579582545235424No Hit
GGGAGGACAGATTCTTTTTATGATTCCTAAAGTGGAAGAAATAAAGTATC1110.18579582545235424No Hit
GGGACTCATGATGCACTGTGTAGCAATCAGCCCCAGCATTTTGGTGATGG1100.18412198918701772No Hit
GGGGGGATGTGTTTTTGACAGGATATGTAACACAGTGTGATTTATAACCA1090.1824481529216812No Hit
GGGATAACTGGAGGTTGCAGGTCAATGACTGATCTTAATTGGGAAGAACA1080.18077431665634466No Hit
GGGGAATGTGCACTTGAACAATGAAGTTGGTGACAAGCATTACTGTACTC1060.17742664412567163No Hit
GGGACTGAGAAGGCTCACGAAGGGCATCCGCAATGTTGGTTTCACTGAGA1030.17240513532966206No Hit
GGGAGATATAAGATTTCAGTTCTCAGTGAGTCTAAGTGACAGAAGGAATG1030.17240513532966206No Hit
GGGGATAACTGGAGGTTGCAGGTCAATGACTGATCTTAATTGGGAAGAAC1010.169057462798989No Hit
GGGAGACTGCTGTCTGCACAAGAAGAAGACTTACAGATGCTTAAGGAGGA1010.169057462798989No Hit
GGGGAGGAAGCTACATACCTACATTTGGAAGAGGAACCAGCCTTATTGTT980.1640359540029794No Hit
GGGGGGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCA960.16068828147230638No Hit
GGGAAAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGG950.15901444520696983No Hit
GGGAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTTC940.15734060894163335No Hit
GGGGGCACTGCCAGTAAACTCACCTTTGGGACTGGAACAAGACTTCAGGT940.15734060894163335No Hit
GGGAGGAAGCTACATACCTACATTTGGAAGAGGAACCAGCCTTATTGTTC930.1556667726762968No Hit
GGGGAGTTGCTGCTGGGCTCATTGCAGCTCAGACACAGCAAAAGAGCCTA920.15399293641096026No Hit
GGGGAGGCCAGTGTTGTGGGCTTCCTGCTGGGACTGAGAAGGCTCACGAA900.15064526388028723No Hit
GGGGACACTCATGATGCACTGTGTAGCAATCAGCCCCAGCATTTTGGTGA890.1489714276149507No Hit
GGGAGATTTGCAGCTTTCTAGGCAGGAGACAAGACAATCTGCATCTTCAC870.14562375508427766No Hit
GGGGTAGAGCAGACTGTGGCTTCACCTCCGAGTCTTACCAGCAAGGGGTC870.14562375508427766No Hit
GGGTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAG860.14394991881894112No Hit
GGGGGAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTT840.1406022462882681No Hit
GGGAATGAGCAGTTCTTCGGGCCAGGGACACGGCTCACCGTGCTAGAGGA840.1406022462882681No Hit
GGGGAACAATGACATGCGCTTTGGAGCAGGGACCAGACTGACAGTAAAAC840.1406022462882681No Hit
GGGAAAACAGCCTTACAAAGACATCCAGCTCTAAGGAGCTCAAAACATCC820.13725457375759503No Hit
GGGATGCCCCAACGTGACAACAGCTTGGAGAGGGGTGGGTACTGGAGAAG820.13725457375759503No Hit
GGGAAGACCAGCCCCTTCGCCAAACAGCCTTACAAAGACATCCAGCTCTA820.13725457375759503No Hit
GGGGACTGAACTTTGAGCTTCAGGCAGCACAACTCACATTTGTGCAAAGA820.13725457375759503No Hit
GGGGGAACTAAGATACAGAAGTGGCGCCTCTGAGAAAAGAAGGTTGGAAT800.13390690122692198No Hit
GGGAGCTTTTGGCTATACATGCCGGGTAAACTCATCCACCTCTCTGTCAG800.13390690122692198No Hit
GGGAGTCACTGCATGTTCAGGAGGGAGACAGCACCAATTTCACCTGCAGC800.13390690122692198No Hit
GGGGAGGCTTTGTCGGGTGGAGCTGATTGGTTGCAGGAGCAGCAACAGTT790.13223306496158546No Hit
GGGAGACAACCTTCACCACCAGGCTGGGACAGCGCCATGGGGACCCAGGG790.13223306496158546No Hit
GGGGGTTTTTTGCCCAATGCCCTCCACAAAGCCAGCGTTTGAAGGAAAAC790.13223306496158546No Hit
GGGACACTAAAGATGAACTATTCTCCAGGCTTAGTATCTCTGATACTCTT780.13055922869624892No Hit
GGGACAATGAGCAGTTCTTCGGGCCAGGGACACGGCTCACCGTGCTAGAG770.1288853924309124No Hit
GGGAGTGTGATTTATAACCAGGGAGGAAAGCTTATCTTCGGACAGGGAAC770.1288853924309124No Hit
GGGGACAGTGTGATTTATAACCAGGGAGGAAAGCTTATCTTCGGACAGGG770.1288853924309124No Hit
GGGGACAGGATATGTAACACAGTGTGATTTATAACCAGGGAGGAAAGCTT760.1272115561655759No Hit
GGGAAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGC760.1272115561655759No Hit
GGGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACG760.1272115561655759No Hit
GGGGATAACCAGGGAGGAAAGCTTATCTTCGGACAGGGAACGGAGTTATC760.1272115561655759No Hit
GGGAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAACAAGGTGTTCC760.1272115561655759No Hit
GGGAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCT750.12553771990023935No Hit
GGGGAAAGCAGATTCTTTTTATGATTTTTAAAGTAGAAATATCCATTCTA750.12553771990023935No Hit
GGGGTAGAGCAGACTGTGGCTTTACCTCGGTGTCCTACCAGCAAGGGGTC750.12553771990023935No Hit
GGGCAGGAACACCAGTGACTCTGAGATGTCACCAGACTGAGAACCACCGC740.12386388363490282No Hit
GGGATCCTTCCCTGACCCTGCCATGGGCACCAGGCTCCTCTGCTGGGCGG740.12386388363490282No Hit
GGGAGCCTTACAAAGACATCCAGCTCTAAGGAGCTCAAAACATCCTGAGG730.12219004736956632No Hit
GGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAACAAGGTGTT720.12051621110422978No Hit
GGGGAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGC720.12051621110422978No Hit
GGGGGTAGAGGAGTTTGACGCTGTGTGGAATATGGAAACAAACTGGTCTT710.11884237483889325No Hit
GGGAGAAGCCTCACACAGCCCAGTAACTTTGCTAGTACCTCTTGAGTGCA710.11884237483889325No Hit
GGGAGATGCAGTGCTTCCTGTCCCTCTGTGCCATGGGCCCCGGGCTCCTC700.11716853857355675No Hit
GGGAGATTTGCAGCTTTCTAGGCAGGAGATAAGACAATCTGCATCTTCAC700.11716853857355675No Hit
GGGGACAGGAGGGATGGCCATGCTCCTGGGGGCATCAGTGCTGATTCTGT700.11716853857355675No Hit
GGGGAGAATGATGGTTTTTGCAATGACTTAGAACACTGTGTATCTAACTT700.11716853857355675No Hit
GGGATGAAAAGGATGTGTGGGCTTCTGGGTGTTTTTGACTGACTAAGAAA700.11716853857355675No Hit
GGGGAGAAGTAACTCTTATAACTGGAGGTTGCAGGTCAATGACTGATCTT700.11716853857355675No Hit
GGGGGATTCTCAGCTGAGAGGCCTGATGGATCATTCTCTACTCTGAAGAT700.11716853857355675No Hit
GGGGTTGAGCTTCCTATCACAGTGGAACACCGGTAACCAGTTCTATTTTG690.1154947023082202No Hit
GGGGAGTATAGGCTCCCTAGACCTGCTCCCCAGCTGTGGTGTAAAAATGG690.1154947023082202No Hit
GGGACAGTCAGACTAACCTCTGCCACCTGCGCTTCCTGCCGCTGCCCAGT690.1154947023082202No Hit
GGGGAGTTCTCAGTGAGTCTAAGTGACAGAAGGAATGGAGACCCTCTTGG680.11382086604288368No Hit
GGGGGCATTTTGGCCATGGCTTTGCAGAGCACTCTGGGGGCGGTGTGGCT680.11382086604288368No Hit
GGGGGAGACACATAACACTGTGACTACCTCAGGAACCTACAAATACATCT670.11214702977754716No Hit
GGGAGGAGGACCAAGCCCTGAGCACAGACACAGTGCTGCCTGCCCCTTTG660.11047319351221063No Hit
GGGAGAGGAGAGAAGAGACAACCTGATGATAGAAGTAACTCTTATAACTG660.11047319351221063No Hit
GGGGGGACTTGGGGAGGCAGGAGTGGAAGGCAGCAGGTCGCGGTTTTCCT660.11047319351221063No Hit
GGGGATAGTGTTTCTTCTGGTTCTGCAAGGCAACTGACCTTTGGATCTGG650.1087993572468741No Hit
GGGGGTTGAGCTTCCTATCACAGTGGAACACCGGTAACCAGTTCTATTTT650.1087993572468741No Hit
GGGGATCTGTGACTGAGGAGCCTTGCTCCATTTCAGGTCTTCTGTGATTT640.10712552098153759No Hit
GGGGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGCT640.10712552098153759No Hit
GGGAGAGTGTGTATTCAGGAGGAGGTGCTGACGGACTCACCTTTGGCAAA640.10712552098153759No Hit
GGGAAAACAGAATGGCTTTTTGGCTGAGAAGGCTGGGTCTACATTTCAGG620.10377784845086453No Hit
GGGGGAGTTTGACGCTGTGTGGAATATGGAAACAAACTGGTCTTTGGCGC610.10210401218552802No Hit
GGGACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCT610.10210401218552802No Hit
GGGAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCC610.10210401218552802No Hit
GGGATGGCTGGAGATTGCAGGTTTATGACTGATCCTATTTGGGAAGAACA610.10210401218552802No Hit
GGGATTACAGTGTGAATTCTGGGGGTTACCAGAAAGTTACCTTTGGAACT610.10210401218552802No Hit
GGGGAGTTACCAACTCACTTTCGGGAAGGGGACCAAACTCTCGGTCATAC610.10210401218552802No Hit
GGGAGGCAGACTGGATGTGTTTTTGACAGGATATGTAACACAGTGTGATT600.10043017592019149No Hit
GGGGATTGCAGACACCCAGACTGGGGACTCAGCTATCTACTTCTGTGCAG600.10043017592019149No Hit
GGGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAACAAGGTGT600.10043017592019149No Hit
GGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGTCTGG600.10043017592019149No Hit
GGGAGAATAAAAATGAAAAAGCATCTGACGACCTTCTTGGTGATTTTGTG600.10043017592019149No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)