FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004953587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59743
Sequences flagged as poor quality0
Sequence length35-300
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACGTTTTTCA12002.0086035184038296No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACCTTGTTCA6641.1114272801834524No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA5840.9775203789565305No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTNGGAACACGTTTTTCA5430.908893092077733No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNGGGTGGGAACACGTTTTTCA4960.8302227876069163No Hit
TGCTTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACGTTTTTCA4940.8268751150762431No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNNGGTGGGAACACGTTTTTCA4920.8235274425455703No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTNGGGTGGGAACACGTTTTTCA4860.8134844249535511No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA4810.8051152436268685No Hit
TGCTTCTGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA4100.6862728687879751No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTGGGTTTCA3510.5875165291331202No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA3310.5540398038263897No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTNGGGTGGGAACACCTTGTTCA3110.5205630785196592No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA2880.4820648444169191No Hit
TGCTTCNGANNGCTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA2810.4703479905595635No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTNGGAACACGTTTTTCA2730.4569573004368712No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTNGGAACACCTTGTTCA2710.4536096279061982No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACGTTTTTCA2660.4452404465795156No Hit
TGCTNCNGANNGCTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA2590.4335235927221599No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTGGGAACACGTTTTTCA2560.42850208392615036No Hit
TGCTTCNGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA2540.4251544113954773No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNNGGTGGGAACACCTTGTTCA2510.42013290259946773No Hit
TGCTTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACCTTGTTCA2480.41511139380345813No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTNGGGTNGGAACACGTTTTTCA2460.41176372127278515No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACGTTTTTCA2440.40841604874211207No Hit
TGCTTCNGANNGNTCAAACACANNNANCTCGGGTNGGAACACGTTTTTCA2420.405068376211439No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNGGGTGGGAACACCTTGTTCA2400.40172070368076596No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACGTTTTTCA2330.39000384982341024No Hit
TGCTTCTGANNGNTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA2290.3833085047620642No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNGGGTGGGAACACGTTTTTCA2280.38163466849672767No Hit
TGCTTCTGANNGNTCAAACACAGCNACCTNGGGTGGGAACACGTTTTTCA2250.37661315970071807No Hit
CGGATGGTTNNGNTAAGGTTGTNTNGTAGNAAGGTGGAGTGGGTTTGGGG2230.37326548717004504No Hit
TGCNTCTGANNGNTCAAACACANNNANCTCGGGTGGGAACACGTTTTTCA2170.3632224695780259No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACGTTTTTCA2150.3598747970473528No Hit
TGCTNCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA2110.35317945198600675No Hit
TGCTTCTGANNGNTCAAACACANCGACCTNGGGTGGGAACACGTTTTTCA2090.34983177945533367No Hit
TGCTNCNGATNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA2040.34146259812865104No Hit
CGGATGGTTNNGNACAACTTATNGNAAAGNTAAAGGAAACCCCAACATGC2010.3364410893326415No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCAGGCCTGA1780.2979428552299014No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATACGGATGAA1720.28789983763788224No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTGGGTTTCA1620.27116147498451704No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATTGGGTTTCA1570.2627922936578344No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATTGGGTTTCA1550.2594446211271613No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATTGGGTTTCA1510.25274927606581526No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATTGGGTTTCA1440.24103242220845955No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACCTTGTTCA1410.23601091341245No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATTGGGTTTCA1410.23601091341245No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATTGGGTTTCA1380.2309894046164404No Hit
TGCTTCTGANNGNTCAAACACANCGACCTNGGGTGGGAACACCTTGTTCA1340.22429405955509432No Hit
TGCTTCNGANNGNTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA1320.22094638702442126No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTGGGAACACCTTGTTCA1320.22094638702442126No Hit
TGCTNCNGANNGCTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA1320.22094638702442126No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACCTTGTTCA1310.21927255075908475No Hit
TGCTNCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA1300.2175987144937482No Hit
TGCTTCNGANNGCTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA1290.21592487822841172No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTNGGGTNGGAACACCTTGTTCA1280.21425104196307518No Hit
TGCTTCNGANNGNTCAAACACANNNANCTCGGGTNGGAACACCTTGTTCA1270.21257720569773866No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTNGGAACACCTTGTTCA1230.20588186063639258No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATAGGGCTGGA1220.20420802437105603No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACCTTGTTCA1220.20420802437105603No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACCTTGTTCA1200.20086035184038298No Hit
TGCTTCTGANNGNTCAAACACAGCNACCTNGGGTGGGAACACCTTGTTCA1180.1975126793097099No Hit
TGCNTCTGANNGNTCAAACACANNNANCTCGGGTGGGAACACCTTGTTCA1170.1958388430443734No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNGGGTGGGAACACCTTGTTCA1130.1891434979830273No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGTTTTA1070.17910048039100815No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGAATGA1060.17742664412567163No Hit
GTCTCTCAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTGGGTTTCA1030.17240513532966206No Hit
CGGANGNTTNNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC980.1640359540029794No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGTTTAA970.16236211773764292No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCAGGTAAAA930.1556667726762968No Hit
TGCTNCNGATNGNTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA920.15399293641096026No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCGAGCGTGA920.15399293641096026No Hit
CGGANGNTTNNGNTAAGGTTGTCNGGNAGTAAGGTGGAGTGGGTTTGGGG890.1489714276149507No Hit
CGGNTGGTTNNGNTAAGGTTGTNNNGNAGNAAGGTGGAGTGGGTTTGGGG890.1489714276149507No Hit
CGGANGNTTNNGNTAAGGTTGTCNGGNAGTAAGGNGGAGTGGGTTTGGGG890.1489714276149507No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNNTTCTGGATATTGGGTTTCA880.14729759134961418No Hit
CGGATGNTTNNGNTAAGGTTGTCNGGNAGNAAGGTGGAGTGGGTTTGGGG870.14562375508427766No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGTATGA870.14562375508427766No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCAGGCCAGA870.14562375508427766No Hit
GTCTCTNAGNNGGTACACGGCAGNNTNAGGGTTCTGGATATTGGGTTTCA860.14394991881894112No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATACGGATGAA860.14394991881894112No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCTGGCTTTA860.14394991881894112No Hit
CGGATGGTTNNGNTAAGGTTGTNNNGNAGNNAGGTGGAGTGGGTTTGGGG850.1422760825536046No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATTGGGTTTCA850.1422760825536046No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATCAGGCCTGA850.1422760825536046No Hit
GTCNCTCAGNNGNTACACGGCANGNTCAGNNTTCTGGATATTGGGTTTCA820.13725457375759503No Hit
GTCTNTNAGNNGNTACACGGCAGNNTNAGNGTTCNGGATATTGGGTTTCA810.13558073749225852No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATCAGGCCTGA810.13558073749225852No Hit
CGGATGGTTNNGNTAAGGTTGTNTNGNAGNNAGGTGGAGTGGGTTTGGGG800.13390690122692198No Hit
GTCNCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTGGGTTTCA800.13390690122692198No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNGTTCTGGATATTGGGTTTCA790.13223306496158546No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA790.13223306496158546No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGNGTTCNGGATATTGGGTTTCA780.13055922869624892No Hit
CGGANGNTTNNGNACAACTTATANAANAGGTAAANGAAACCCCAACATGC780.13055922869624892No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGCTGGA780.13055922869624892No Hit
CGGATGGTTNNGNTAAGGTTGTCNNGNAGTAAGGTGGAGTGGGTTTGGGG770.1288853924309124No Hit
CGGATGGTTNNGNACAACTTATNNNANAGNNAAAGGAAACCCCAACATGC760.1272115561655759No Hit
CGGATGGTTNNGNACAACTTATNGNANAGNNAAAGGAAACCCCAACATGC760.1272115561655759No Hit
GTCTCTNAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATTGGGTTTCA750.12553771990023935No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATCAGGCCTGA740.12386388363490282No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATACGGATGAA740.12386388363490282No Hit
CGGNTGGTTNNGNACAACTTATNNNANAGNTAAAGGAAACCCCAACATGC740.12386388363490282No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTGAGTTCCA730.12219004736956632No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATACGGATGAA730.12219004736956632No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATACGGATGAA730.12219004736956632No Hit
GTCTCTNAGNNGNTACACGGCANNNTNAGGGTTCNGGATATTGGGTTTCA710.11884237483889325No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGCTTTAA710.11884237483889325No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTGGGCTTCA700.11716853857355675No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGAAAGA700.11716853857355675No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGGGTTCTGGATATTGGGTTTCA700.11716853857355675No Hit
GTCTNTNAGNNGGTACACGGCAGNGTNAGGGTTCTGGATATTGGGTTTCA690.1154947023082202No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGATGGA690.1154947023082202No Hit
CGGATGNTTNNGNACAACTTATANAANAGNTAAAGGAAACCCCAACATGC680.11382086604288368No Hit
GTCTNTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATTGGGTTTCA680.11382086604288368No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGGATGA670.11214702977754716No Hit
GTCTNTNAGNNGNTACACGGCAGNNTNAGNGTTCTGGATATTGGGTTTCA670.11214702977754716No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATACGGATGAA650.1087993572468741No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGCTTCA650.1087993572468741No Hit
GTCTCTCAGNNGNTACACGGCAGGNTCAGNGTTCTGGATATTGGGTTTCA650.1087993572468741No Hit
GTCTCTCAGNNGNTACACGGCANGGTCAGNGTTCTGGATATTGGGTTTCA650.1087993572468741No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGGTTGA640.10712552098153759No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATACGGATGAA640.10712552098153759No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATCAGGCCTGA640.10712552098153759No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATCAGGCCTGA630.10545168471620106No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGTTGCA630.10545168471620106No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTGGAACTCA620.10377784845086453No Hit
GTCTNTNAGCNGNTACACGGCAGNGTNAGGGTTCTGGATATTGGGTTTCA620.10377784845086453No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATCAGGCCTGA610.10210401218552802No Hit
CGGATGGTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC600.10043017592019149No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)