FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004953592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67911
Sequences flagged as poor quality0
Sequence length35-300
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTT5990.8820367834371456No Hit
GGGGGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGC5770.849641442476182No Hit
GGGAGGACAGATTTCTTTTATGATTCCTACAGCAGAAAAATGAGAAACGT5480.8069384930276391No Hit
GGGGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCC5400.7951583690418342No Hit
GGGAGAGGTCCTGTTTCCCTGACAATCATGACTTTCAGTGAGAACACAAA4760.7009173771553945No Hit
GGGGAAGTCAACTTCTGGGAGCAGATCTCTGCAGAATAAAAATGAAAAAG4720.695027315162492No Hit
GGGAGATATACAGCAACTCTGGATGCAGACACAAAGCAAAGCTCTCTGCA4500.6626319742015284No Hit
GGGGAGGACAGATTTCTTTTATGATTCCTACAGCAGAAAAATGAGAAACG4430.6523243657139491No Hit
GGGAGCGCCCGAATCTGGCTTGGCGGAATATCTCTTTGACAAGCACACCC4250.625819086745888No Hit
GGGGACACTAAAGATGAACTATTCTCCAGGCTTAGTATCTCTGATACTCT3880.5713360133115402No Hit
GGGAGGAGGAAGCTACATACCTACATTTGGAAGAGGAACCAGCCTTATTG3810.5610284048239608No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG3670.5404131878488021No Hit
GGGGAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAA3480.5124353933825153No Hit
GGGAAGTCAACTTCTGGGAGCAGATCTCTGCAGAATAAAAATGAAAAAGC3460.5094903623860642No Hit
GGGATAACTGGAGGTTGCAGGTCAATGACTGATCTTAATTGGGAAGAACA3120.45942483544639307No Hit
GGGGATGAAAAGGATGTGTGGGCTTCTGGGTGTTTTTGACTGACTAAGAA3090.45500728895171616No Hit
GGGGAGATCTCTGCAGAATAAAAATGAAAAAGCATCTGACGACCTTCTTG2910.4285020099836551No Hit
GGGGATTTTGGCCATGGCTTTGCAGAGCACTCTGGGGGCGGTGTGGCTAG2870.42261194799075263No Hit
GGGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGT2740.40346924651381955No Hit
GGGAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCT2670.3931616380262402No Hit
GGGAGATCTCTGCAGAATAAAAATGAAAAAGCATCTGACGACCTTCTTGG2660.3916891225280146No Hit
GGGGAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGC2640.38874409153156336No Hit
GGGGGATCTTAATTGGGAAGAACAAGGATGACATCCATTCGAGCTGTATT2630.3872715760333377No Hit
GGGATATCTCTAGGTCTTAGGTATGAAAAGGATGTGTGGGCTTCTGGGTG2460.3622388125635022No Hit
GGGATGAAAAGGATGTGTGGGCTTCTGGGTGTTTTTGACTGACTAAGAAA2450.36076629706527663No Hit
GGGAGATCAGAAGAGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGCA2440.359293781567051No Hit
GGGAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTTC2420.3563487505705998No Hit
GGGAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAA2380.35045868857769724No Hit
GGGGACTGTGATTTCTTCATGTTAAGGATCAAGACCATTATTTGGGTAAC2330.3430961110865692No Hit
GGGGAGATATAAGATTTCAGTTCTCAGTGAGTCTAAGTGACAGAAGGAAT2310.34015108009011796No Hit
GGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAACAAGGTGTT2300.33867856459189233No Hit
GGGGAGAATGTGGAGCAGCATCCTTCAACCCTGAGTGTCCAGGAGGGAGA2190.3224808941114105No Hit
GGGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCT2150.316590832118508No Hit
GGGGGACTCAGCAATGTACTTCTGTGCAATGAGAGAGGGCGATACTGGAG2080.3062832236309287No Hit
GGGAGAAACACAGTGGAAGATTAAGAGTCACGCTTGACACTTCCAAGAAA1870.2753603981681907No Hit
GGGATTTTGGCCATGGCTTTGCAGAGCACTCTGGGGGCGGTGTGGCTAGG1850.2724153671717395No Hit
GGGGAGATCAGAAGAGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGC1850.2724153671717395No Hit
GGGAGATATAAGATTTCAGTTCTCAGTGAGTCTAAGTGACAGAAGGAATG1800.2650527896806114No Hit
GGGGAGGAGGAAGCTACATACCTACATTTGGAAGAGGAACCAGCCTTATT1790.2635802741823858No Hit
GGGAGGGAGACAGCGCTGTTATCAAGTGTACTTATTCAGACAGTGCCTCA1720.25327266569480644No Hit
GGGGAGCCCAGAGAAGTCTGTTCCAGGACGGGCTCTTTCAGGAGCAGCTA1680.24738260370190396No Hit
GGGGATAACTGGAGGTTGCAGGTCAATGACTGATCTTAATTGGGAAGAAC1660.24443757270545272No Hit
GGGGAGAATGCTTACTACAGAGACACCAGCCCCAAGCTAGGAGATCCTGC1660.24443757270545272No Hit
GGGGGACTGACTAAGAAACACTGTGGGATGGATAGCAGCTATAAATTGAT1590.2341299642178734No Hit
GGGGGATGATAGAAGTAACTCTTATAACTGGAGGTTGCAGGTCAATGACT1580.23265744871964777No Hit
GGGAGAAGTAACTCTTATAACTGGAGGTTGCAGGTCAATGACTGATCTTA1580.23265744871964777No Hit
GGGACACTAAAGATGAACTATTCTCCAGGCTTAGTATCTCTGATACTCTT1520.22382235573029405No Hit
GGGAGTTCTTCGGGCCAGGGACACGGCTCACCGTGCTAGAGGACCTGAAA1370.20173462325690977No Hit
GGGGAGGACAGATTCTTTTTATGATTCCTAAAGTGGAAGAAATAAAGTAT1360.20026210775868414No Hit
GGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACAAAGACATCCAGCTC1350.19878959226045856No Hit
GGGGAGAATAAAAATGAAAAAGCATCTGACGACCTTCTTGGTGATTTTGT1350.19878959226045856No Hit
GGGAGAATAAAAATGAAAAAGCATCTGACGACCTTCTTGGTGATTTTGTG1340.19731707676223292No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1330.1958445612640073No Hit
GGGGGGCACTGCCAGTAAACTCACCTTTGGGACTGGAACAAGACTTCAGG1320.19437204576578168No Hit
GGGGGTTGAATACACAAGTCAACTTCTGGGAGCAGATCTCTGCAGAATAA1290.18995449927110483No Hit
GGGACAATGAGCAGTTCTTCGGGCCAGGGACACGGCTCACCGTGCTAGAG1290.18995449927110483No Hit
GGGAGTCTTTCTTCTGGTACAGACAGGATTGCAGGAAAGAACCTAAGTTG1290.18995449927110483No Hit
GGGAAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGT1290.18995449927110483No Hit
GGGGATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCAC1280.18848198377287922No Hit
GGGAGCCCCAGCATTTTGGTGATGGGACTCGACTCTCCATCCTAGAGGAC1220.1796468907835255No Hit
GGGATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTTCCCACC1210.1781743752852999No Hit
GGGGGACTTGATTATGAAATGCTACCAGTTTGAAGCAGTCACTGAAAAAC1170.1722843132923974No Hit
GGGGACACCAGCTCCTCTGGGGTTTGCCACACTCATGATGCACTGTGTAG1160.1708117977941718No Hit
GGGGAAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTG1150.16933928229594616No Hit
GGGGGCCATATCTCTAGGTCTTAGGTATGAAAAGGATGTGTGGGCTTCTG1130.16639425129949492No Hit
GGGAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTTCC1120.16492173580126931No Hit
GGGGAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTT1100.16197670480481807No Hit
GGGGGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCAT1100.16197670480481807No Hit
GGGGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCA1090.16050418930659247No Hit
GGGACAAAGACATCCAGCTCTAAGGAGCTCAAAACATCCTGAGGACAGTG1060.1560866428119156No Hit
GGGAGAGTGTTTATCTTGTGAGCCATTCTCCATATTTCAGATATAAGATT1020.15019658081901313No Hit
GGGGATATCTCTAGGTCTTAGGTATGAAAAGGATGTGTGGGCTTCTGGGT1010.1487240653207875No Hit
GGGAGGACAGATTCTTTTTATGATTCCTAAAGTGGAAGAAATAAAGTATC1000.14725154982256186No Hit
GGGATTGCAGACACCCAGACTGGGGACTCAGCTATCTACTTCTGTGCAGA990.14577903432433625No Hit
GGGAGTATAGGCTCCCTAGACCTGCTCCCCAGCTGTGGTGTAAAAATGGA970.14283400332788504No Hit
GGGGAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGC970.14283400332788504No Hit
GGGGAAGGTTTTTCGTAATGATGCCTGTGGTAGTGCTTTGACAGCACAAC940.13841645683320816No Hit
GGGGAGAAGTAACTCTTATAACTGGAGGTTGCAGGTCAATGACTGATCTT940.13841645683320816No Hit
GGGGTAGAGCAGACTGTGGCTTCACCTCCGAGTCTTACCAGCAAGGGGTC940.13841645683320816No Hit
GGGGAGAAGAGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGCATGGC940.13841645683320816No Hit
GGGGATTGCAGACACCCAGACTGGGGACTCAGCTATCTACTTCTGTGCAG920.13547142583675692No Hit
GGGAGGAAAAGATAGGCTCAGGAAAGAGAGGAAGGGTGTGCCCTCTGTCT910.1339989103385313No Hit
GGGAGCCCAGAGAAGTCTGTTCCAGGACGGGCTCTTTCAGGAGCAGCTAA910.1339989103385313No Hit
GGGAGCATTTTGGTGATGGGACTCGACTCTCCATCCTAGAGGACCTGAAC910.1339989103385313No Hit
GGGGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGCT900.1325263948403057No Hit
GGGGAGAAAGACTAGGGATTCACCCAGTAAAGAGAGCTCATCTGTGACTG900.1325263948403057No Hit
GGGAAAGACATCCAGCTCTAAGGAGCTCAAAACATCCTGAGGACAGTGCC880.12958136384385444No Hit
GGGAGTTGCTGCTGGGCTCATTGCAGCTCAGACACAGCAAAAGAGCCTAG870.12810884834562883No Hit
GGGGAGGTATGAAAAGGATGTGTGGGCTTCTGGGTGTTTTTGACTGACTA870.12810884834562883No Hit
GGGGGAGAGTACTGTTCCCCTATCACCGATGCACAGACCCAGAAGACCCC870.12810884834562883No Hit
GGGAGATTTGCAGCTTTCTAGGCAGGAGACAAGACAATCTGCATCTTCAC860.12663633284740322No Hit
GGGGATCTTAATTGGGAAGAACAAGGATGACATCCATTCGAGCTGTATTT860.12663633284740322No Hit
GGGGATTGCAGCTCAGACACAGCAAAAGAGCCTAGAACCTGGGTCCTAGT820.12074627085450075No Hit
GGGGACCATTATTTGGGTAACACACTAAAGATGAACTATTCTCCAGGCTT820.12074627085450075No Hit
GGGGAGAGCAGGAGACTTTTCACTCTGCAGGGGAGCGCTGTCAGCATGAC810.11927375535627513No Hit
GGGGACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGC800.1178012398580495No Hit
GGGGAGTATAGGCTCCCTAGACCTGCTCCCCAGCTGTGGTGTAAAAATGG800.1178012398580495No Hit
GGGGAGCATTTTGGTGATGGGACTCGACTCTCCATCCTAGAGGACCTGAA790.11632872435982389No Hit
GGGAGGAAACATTTTTGTATCATGGTGTAACATTGTGGGGACTAGCGGGA790.11632872435982389No Hit
GGGACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTTCCCA790.11632872435982389No Hit
GGGAGAAGAGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGCATGGCA780.11485620886159827No Hit
GGGGCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTG780.11485620886159827No Hit
GGGAAGGAGGTGCACAGTGGGGTCAGCACGGACCCGCAGCCCCTCAAGGA770.11338369336337266No Hit
GGGGACTGACTAAGAAACACTGTGGGATGGATAGCAGCTATAAATTGATC750.1104386623669214No Hit
GGGAGTGCTTTGCCAACCTGGCCTGTTTGATCTGGTTTTTGTTGTTGAGC750.1104386623669214No Hit
GGGGAGCTTTTGGCTATACATGCCGGGTAAACTCATCCACCTCTCTGTCA730.10749363137047019No Hit
GGGGGTTCATATGTAAAATGAAGGGTCTGTGGAAGGACATGAATAAAGCA730.10749363137047019No Hit
GGGAAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGC720.10602111587224455No Hit
GGGGATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTTCCCAC720.10602111587224455No Hit
GGGATTATTTGGGTAACACACTAAAGATGAACTATTCTCCAGGCTTAGTA720.10602111587224455No Hit
GGGCAGTTCTTCGGGCCAGGGACACGGCTCACCGTGCTAGAGGACCTGAA710.10454860037401893No Hit
GGGATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACT710.10454860037401893No Hit
GGGGGGACTTGGGGAGGCAGGAGTGGAAGGCAGCAGGTCGCGGTTTTCCT710.10454860037401893No Hit
GGGGACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGC690.1016035693775677No Hit
GGGGGCATTTTGGCCATGGCTTTGCAGAGCACTCTGGGGGCGGTGTGGCT690.1016035693775677No Hit
GGGGAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATG690.1016035693775677No Hit
GGGGAATGTGCACTTGAACAATGAAGTTGGTGACAAGCATTACTGTACTC690.1016035693775677No Hit
GGGAGAGCGGGCTGAATGCAATGGAGTGTGCATTACATTTGGAAAAAAAT680.10013105387934207No Hit
GGGAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCTTGGCTATGT680.10013105387934207No Hit
GGGGACAGAAGTGGCGCCTCTGAGAAAAGAAGGTTGGAATTATCGTAATT680.10013105387934207No Hit
GGGACACTCATGATGCACTGTGTAGCAATCAGCCCCAGCATTTTGGTGAT680.10013105387934207No Hit
GGGATATAACTGGAGGTTGCAGGTCAATGACTGATCTTAATTGGGAAGAA680.10013105387934207No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)