FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004953594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45394366
Sequences flagged as poor quality0
Sequence length21
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGG1105460.24352361259985436No Hit
GCAGCGTAAACCGGGGGGGGT537380.11838032940034893No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCTC8826150.014.9897951
TACTACG4672700.014.982931
TAGCTCC8881300.014.981922
CTGCGCA4124900.014.9747551
GCAGCGT7605000.014.9730351
ACTACGC4728800.014.970632
CGCTCAG3058250.014.9676951
CAGCGTA7679100.014.9668372
AGCTCCT8916250.014.9635513
GTCTTAG4460250.014.9621751
TCTTAGG4477650.014.9544292
GAGCGCT4400150.014.9543011
CTACGCC4732000.014.9539913
AGCGCTA4426550.014.9515352
AGGCTCC3920550.014.9450191
ACTGATC1455500.014.9443131
AGCGTAA7696500.014.9424373
GCGCTAC4444950.014.9394013
TACGCCC4721600.014.9371954
CTCCTTA8934050.014.936815
Produced by FastQC (version 0.11.5)