FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004953595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45394366
Sequences flagged as poor quality0
Sequence length62
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA818250.034.1615141
GGTATCA268400.033.856681
GCCGTAT40300.029.47856156
GTCGCCG146350.024.9266456
TCAACGC1143700.023.885614
TATCAAC1169650.023.587212
ATCAACG1184250.023.1228313
CAACGCA1202150.022.8011255
GGTCGCC167300.021.87134455
CGCCGTA56200.021.63623255
CTTGCGG57050.020.5628833
CCGATCA127000.019.80314449
TGCGGGT59900.019.4921935
TTGCGGG60200.019.39494534
GCCGATC130450.019.23562448
CGATCAC131500.019.1057350
AACGCAG1442850.019.0913166
ATCGCCG135000.018.33627345
TCGCCGA138000.018.28317846
TCTTGCG62850.018.21971932
Produced by FastQC (version 0.11.5)