FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004953596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37693861
Sequences flagged as poor quality0
Sequence length21
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGG1031460.27364137624426427No Hit
GCAGCGTAAACCGGGGGGGGT484590.1285593959186086No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATC4389300.014.9837191
TACTACG3375700.014.9764511
CTGCGCA3846150.014.9733361
CGCTCAG2830050.014.9725851
TAGCTCC2960850.014.9681682
GCAGCGT6485000.014.9648751
GTAGCTC2942400.014.9647851
ACTACGC3417950.014.9642492
CAGCGTA6550450.014.9641992
GAGCGCT4521900.014.9587111
AGCGCTA4544150.014.955512
TCTTAGG3754400.014.9524342
GTCTTAG3739450.014.9520581
CTGATCG4407650.014.9504012
AGCGTAA6561400.014.9490013
CTACGCC3421600.014.9435243
GCGCTAC4563150.014.9407973
AGCTCCT2972750.014.9385823
AGGCTCC2776200.014.9295941
TACGCCC3412650.014.9273334
Produced by FastQC (version 0.11.5)