FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004953597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37693861
Sequences flagged as poor quality0
Sequence length62
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTAGCCCTGTGCCCCCTGAAACAGCTGCCACCATCACTCGCAAGAGAATCCCCTCCATC500830.13286778979738903No Hit
CCCTGGGAGACAGTGATAATGAAAGCTAAGCCTCGGGCTAATTTCCCCATAGCCGTGGGGTG495680.13150151957105163No Hit
GTCTAGAGCTTGTCTCAATGGATCTAGAACTTCATCGCCCTCTGATCGCCGATCACCTCTGA454940.12069339354755938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA656150.033.8181841
GGTATCA210800.033.521681
GCCGTAT36000.029.73088856
GTCGCCG151600.026.81731256
GGTCGCC171650.023.7654955
TCAACGC910850.023.7197154
CGCCGTA46500.023.67929355
TATCAAC937650.023.3170722
ATCAACG935400.023.140863
CAACGCA972300.022.2632435
TCGCCGT84500.021.42016856
CCGATCA129250.021.04014849
GCCGATC133950.020.21727248
CGATCAC139000.019.58552750
CGCCGAT139650.019.37086547
ATCGCCG140450.019.33946245
TCGCCGA142800.019.25694846
AACGCAG1142800.018.9844746
CCACGAA131750.018.9577237
TGATCGC144500.018.87523743
Produced by FastQC (version 0.11.5)