FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004953600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13342
Sequences flagged as poor quality0
Sequence length35-300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN649448.67336231449558No Hit
GGGAGCGCCCGAATCTGGCTTGGCGGAATATCTCTTTGACAAGCACACCC179513.453755059211511No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG1841.3791035826712637No Hit
GGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACGA1451.0867935841702894No Hit
GGGGTGACTTCCCTGGTCACCAAGGCAGTGCATGCATGTTGGGGTTTCCT1060.794483585669315No Hit
GGGGAGGCCAGTGTTGTGGGCTTCCTGCTGGGACTGAGAAGGCTCACGAA900.6745615350022486No Hit
GGGAAGATCCCATTCCATTGCATTCCATTACATTCCATTCCCCTGCACTC840.6295907660020986No Hit
GGGGTTTCCTTTACCTTTTCTATAAGTTGTACCAAACCATCCG820.6146005096687154No Hit
GGGATGGTGAGGTTCTCTTCACTCATGGATTTCCTGTCGTTCTATTGGCT760.5696297406685654No Hit
GGGACTGAGAAGGCTCACGAAGGGCATCCGCAATGTTGGTTTCACTGAGA760.5696297406685654No Hit
GGGACAATTCACGTGAAGAGCCTGGCCCAGCACCCAGCTGACACCTCATG730.5471443561684904No Hit
GGGTGACCACGTGACCAACTTGCGCAAGATGGGAGCGCCCGAATCTGGCT680.5096687153350322No Hit
GGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACAT650.4871833308349573No Hit
GGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTG640.4796882026682657No Hit
GGGACATATTTCCTCTTCTTCTACCTTTAGCTTTTATTCCACAGCTCTTG630.472193074501574No Hit
GGGAGGAAGAAAAGGAAAGTGAAGAAGCCATCCTAGCAGATGATGATGAA600.44970769000149907No Hit
GGGATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGG570.4272223055014241No Hit
GGGATTCATCATCTAATTTTGCATATTATGTCTCAAACATTTTCTATTTA540.4047369210013491No Hit
GGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG540.4047369210013491No Hit
GGGGATGCTTGCATGTGTAATCTTACTAAGAGCTAATAGAAAGGCTAGGACCAAACCATCCG500.3747564083345825No Hit
GGGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGT440.3297856393344326No Hit
GGGGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGCT410.3073002548343577No Hit
GGGAGACAATTCACCCCTCCACTTTGGGAATGGGACCAGGCTCACTGTGA410.3073002548343577No Hit
GGGGATATGCCTTCTAATGGCAAAGATTTCTGTAGGACCCAGAGTCTTGT410.3073002548343577No Hit
GGGATTCAATTCCATTCCACTCGGGTTGATTCCATTCCAATCCATTCCAT390.2923099985009744No Hit
GGGGGAATTACTTGCAGGGCTAACCTAGTGCCTATAGCTAAGGCAGGTAC340.2548343576675161No Hit
GGGGGACTCCGCTTTGTACTTCTGTGCTGTGAGAATGGGTCGGACCTTTA330.24733922950082443No Hit
GGGAGAGCGGGCTGAATGCAATGGAGTGTGCATTACATTTGAAAAAAAAT320.23984410133413284No Hit
GGGGATTGCAGCTCAGACACAGCAAAAGAGCCTAGAACCTGGGTCCTAGT310.2323489731674412No Hit
GGGAGTGATTCAGCCACCTACCTCTGTGTGGTGAACACGCTCTCCGGCAA310.2323489731674412No Hit
GGGAGAGCGGGCTGAATGCAATGGAGTGTGCATTACATTTGGAAAAAAAT300.22485384500074954No Hit
GGGACTCGACTCTCCATCCTAGAGGACCTGAACAAGGTGTTCCCACCCGA290.21735871683405789No Hit
GGGGATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTTCCCAC290.21735871683405789No Hit
GGGATGCTTCTGCAGGCTGCCACATTACACTGCTCATGGCCCTTCTTCCC290.21735871683405789No Hit
GGGAACAGTTAGAAGGTGTCCTTAATTGTCCTTTTTGTCATATATTATAT290.21735871683405789No Hit
GGCTCATTCTTTTCATAATTAAAGGCAGAGTACATGTTTTACTTCCAGTA280.2098635886673662No Hit
GGGCAGATTTCACCTCCCCAGAGCTCAGCCACTTAGTGCTGTTACACCTT270.20236846050067456No Hit
GGGGAGAGTGTTTATCTTGTGAGCCATTCTCCATATTTCAGATATAAGAT270.20236846050067456No Hit
GGGGAGCTTTTGCTACACTGGGACTGAGGTGTTCTAAGCCTGGAAAGACA260.1948733323339829No Hit
GGGGGACTGACTAAGAAACACTGTGGGATGGATAGCAGCTATAAATTGAT260.1948733323339829No Hit
GGGTCAAAGTATTTAGCTGACTCGCCACACTCCACGGAAGCAATATGAAA260.1948733323339829No Hit
GGGAGCGCCCGAATCTGGCTTGGCGGAATATCTTTTTGACAAGCACACCC260.1948733323339829No Hit
GGGTCCTTTTTCTGATCCAAGGTCCCATTCGGGATACCACATTACATTTA260.1948733323339829No Hit
GGGAACTTTAAGAAACTTAAGAAATTGAGTATGCCTGGATGCTGTCAGTC250.18737820416729126No Hit
GGGAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTA250.18737820416729126No Hit
GGGAGAGCGGGCTGAATGCAATGGAGTGTGCATTACATTTGGAAAAAAAA250.18737820416729126No Hit
GGGACTAGGAGAGCAGGTGCAAACAACAGCTCTTCAGGCCAAAGCCAGGG250.18737820416729126No Hit
GGGATAACTGGAGGTTGCAGGTCAATGACTGATCTTAATTGGGAAGAACA250.18737820416729126No Hit
GGGACACCCCGCGGGCCTCCCGTTGTCATGCCTTACGGTTTCCAATGCGC240.1798830760005996No Hit
GGGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACG240.1798830760005996No Hit
GGGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTAC240.1798830760005996No Hit
GGGGAGAAAAGAATGATGATATCCTTGAGAGTTTTACTGGTGATCCTGTG230.17238794783390796No Hit
GGGAGTGTAAATACCGGCACTGCCAGTAAACTCACCTTTGGGACTGGAAC230.17238794783390796No Hit
GGGGTAGTGCCTCTGTTTGTCCCTCTGCCCTCCTCTTGTTCCACTCTCTCCTCATACCAAACCATCCG230.17238794783390796No Hit
GGGAAGCTCGAACTGGGCAGAGCCCACTGCAGCGCAGCAAGGCTTGCTGC220.1648928196672163No Hit
GGGGACGAAACCCTCAGCCCATATGAGCGACGCGGCTGAGTACTTCTGTG220.1648928196672163No Hit
GGGGAGACTGAGTTTCCTTCCCTCACCCACATGAAGTGTCTACCTTCTGC220.1648928196672163No Hit
GAGCCACCAGATAAGCAAGGAGGTAATAACAGCTTAAACCAATAGCCACT220.1648928196672163No Hit
GGGTGAGCAGCAAACAGGAGGTGACGCAGATTCCTGCAGCTCTGAGTGTC220.1648928196672163No Hit
GGGACAATGAGCAGTTCTTCGGGCCAGGGACACGGCTCACCGTGCTAGAG220.1648928196672163No Hit
GGGCAGAGTCCGTGGAGCGCGAGATCCAGTACGTCAAGGACCTGCTCATC220.1648928196672163No Hit
GGGGAGATATAAGATTTCAGTTCTCAGTGAGTCTAAGTGACAGAAGGAAT220.1648928196672163No Hit
GGGGGAGGCTGGACCCTCCCAAGAAGCCCCTTTACAGCATGGCCAGCCCC210.15739769150052466No Hit
GGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTAT210.15739769150052466No Hit
GGGGGGTGACCACGTGACCAACTTGCGCAAGATGGGAGCGCCCGAATCTG210.15739769150052466No Hit
GGGAATCCATCCACGGCATGCTTTTGTCCCCAGACATCGAATATATGCAA200.149902563333833No Hit
GGGGAACAATGAAACCAGCAACTCATTCTGTCTTTCTGAATTTGTAATAT200.149902563333833No Hit
GGGGAAGTCTATCCCATTTGATTAAGAATAACAACCTAATCCTCTGTGGA200.149902563333833No Hit
GGGCATCACCATCTTTGAGAAGTCCAGAGTTATCAATGGGTCCAGGATAC200.149902563333833No Hit
GGGACAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATG190.14240743516714136No Hit
GGGGGCGTTTCTACTCGCTGCTGGATCCCTCTTATGCTAAGAACAACATC190.14240743516714136No Hit
GGGGAGCATCTCACAGTGTAAATACCGGCACTGCCAGTAAACTCACCTTT190.14240743516714136No Hit
GGGATAGGCTTTGGGAATGTGCTGCATTGCGGGTCCGGCACTCAAGTGAT190.14240743516714136No Hit
GGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAACCATCCG180.1349123070004497No Hit
GGGGGACTCAGCAATGTACTTCTGTGCAATGAGGGAAAATTCCGGGTATG180.1349123070004497No Hit
GGGGAGAAGACTCAGCCCTGTATCTCTGCGCCAGCAGCCCAACAGAGGAG180.1349123070004497No Hit
GGGGGAACTGGACAGAAAAAAAAAATGAAGAAGCTACTAGCAATGATTCT170.12741717883375805No Hit
GGGGATTTTGGCCATGGCTTTGCAGAGCACTCTGGGGGCGGTGTGGCTAG170.12741717883375805No Hit
GGGGGAAATTCTTTAAACCTAGAATCAGACACAAAAACTGAACTCTGGGT170.12741717883375805No Hit
GGGGAGTTCAGTTCTCAGTGAGTCTAAGTGACAGCAGGACGTAGTACTTT170.12741717883375805No Hit
GGGGACAGGATATGTAACACAGTGTGATTTATAACCAGGGAGGAAAGCTT170.12741717883375805No Hit
GGGATTGCAGGCGTGAGCCACTGCGCCCGGCCACATTCAGTTCTTATCAA170.12741717883375805No Hit
GGGACTGCCAGTAAACTCACCTTTGGGACTGGAACAAGACTTCAGGTCAC160.11992205066706642No Hit
GGGGGCAGTCACCTCTTTCCCTTTCTTTCCAAAATGCACACATCTACTTT160.11992205066706642No Hit
GGGAGGGCCCTCCTCGAGGGAAGCGGGGTTTGCGCCAGGGTCCCCAGGGC160.11992205066706642No Hit
GACCTCCATCCCCATTCCCACAGCAGCCAAAGTGAGCCTTTCAAGCATAAACCAAACCATCCG160.11992205066706642No Hit
GGGGTACATGTGCAGAACATGCAGGTTTGTTACATAGTTATATACGTGCC160.11992205066706642No Hit
GGGAAAAAACATTCAGTACTTCGGCGCCGGGACCCGGCTCTCAGTGCTGG160.11992205066706642No Hit
GGGAATATGAAGGCTGCAGCGATGCCTGCAAGTCAGCTTTTGCTACACTG160.11992205066706642No Hit
GGGTGCAGATTGCAATGCTGTACTTGGTGCATTTGTTTTTCTCAGTAAAA160.11992205066706642No Hit
GGGGAGAACAGCGTCTTTTACCTGTCACCGAAGCCAGGAAGCCCCGTTTG160.11992205066706642No Hit
GGGGACAGGCTGGGCCCACACCCCCATGCCCCACAGGTACCTACTGCGAC160.11992205066706642No Hit
GCGCATTTCCGGCAATGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.11242692250037477No Hit
GGGGAGCACATTCATCTTTGGGAGTGGGACAAGATTATCAGTAAAACCTG150.11242692250037477No Hit
GGGAAGAGATACTTGATAATATAGCTCTCTTGGCTGGAGATTGCAGGTCC140.1049317943336831No Hit
GGGGATTATTGTAAAGCCCCATAGGACTGTGTGAATTATGGAGGAAGCCA140.1049317943336831No Hit
GGGAGTCAGATATGGCAGGAATGTTGGAATTATCAGACCAGGAATTTTAA140.1049317943336831No Hit
GGGAGCTCAGGGCCCCCATTGATTCTACATTATGGTGAGTTGTATAACTA140.1049317943336831No Hit
GGGAGATTTGCAGCTTTCTAGGCAGGAGACAAGACAATCTGCATCTTCAC140.1049317943336831No Hit
GGGAAAAACATTGAAGACGTCATTGCCCAGGGTATTGGCAAGCTTGCCAGTGTACCAGCTGAGAGAC140.1049317943336831No Hit
GGGGGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGC140.1049317943336831No Hit
GGGCTGACACAGGCAAAGCCCTCAACATATAATTATAATAGGTGTCATGT140.1049317943336831No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAACCAAACCATCCG140.1049317943336831No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)