FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004953603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357043
Sequences flagged as poor quality0
Sequence length288-300
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACGTTTTTCA60811.7031562024742117No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA28450.7968227916525461No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTNGGAACACGTTTTTCA25540.7153200034729711No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA25180.7052371843167349No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTNGGGTGGGAACACGTTTTTCA25090.7027164795276759No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNNGGTGGGAACACGTTTTTCA23900.6693871606501178No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNGGGTGGGAACACGTTTTTCA23760.6654660643115815No Hit
TGCTTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACGTTTTTCA23270.6517422271267046No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCAGGCCTGA21560.6038488361345833No Hit
TGCTTCTGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA20940.5864839809210656No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACCTTGTTCA17850.49993978316337245No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGCTTTAA16560.4638096811868599No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGTTTTA14180.39715104343174357No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCAGGTAAAA13540.37922603159843493No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACGTTTTTCA12860.36018070652554457No Hit
TGCTTCNGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA12840.35962054990575365No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACGTTTTTCA12690.355419375257322No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTNGGAACACGTTTTTCA12480.34953773074951755No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACGTTTTTCA12230.3425357730021314No Hit
TGCTTCNGANNGCTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA12080.33833459835369967No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTGGGAACACGTTTTTCA12060.3377744417339088No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTNGGGTNGGAACACGTTTTTCA11990.3358138935646407No Hit
TGCTNCNGANNGCTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA11930.334133423705268No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACGTTTTTCA11850.3318927972261044No Hit
TGCTTCTGANNGNTCAAACACANCGACCTNGGGTGGGAACACGTTTTTCA11810.3307724839865226No Hit
TGCTNCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA11640.32601115271830006No Hit
TGCTTCTGANNGNTCAAACACAGCNACCTNGGGTGGGAACACGTTTTTCA11620.32545099609850914No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCGAGCGTGA11490.32180997806986833No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNGGGTGGGAACACGTTTTTCA11300.3164884901818548No Hit
TGCTTCNGANNGNTCAAACACANNNANCTCGGGTNGGAACACGTTTTTCA11040.3092064541245732No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCTGGTTTTA10890.30500527947614153No Hit
TGCNTCTGANNGNTCAAACACANNNANCTCGGGTGGGAACACGTTTTTCA10640.2980033217287553No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTGGGTTTCA9940.2783978400360741No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATCAGGCCTGA9920.27783768341628323No Hit
TGCTNCNGATNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA9500.26607439440067443No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATACGGATGAA9320.2610329848225564No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGAATGA9100.25487126200485655No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGTTTAA8960.2509501656663203No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATCAGGCCTGA8790.24618883439809772No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATCAGGCCTGA8780.24590875608820226No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATGGGGTTTGA8780.24590875608820226No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATCAGGCCTGA8700.2436681296090387No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATCAGGCCTGA8600.2408673465100842No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA8580.24030718989029332No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATCAGGCCTGA8520.23862672003092064No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATCAGGCCTGA8420.23582593693196618No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGCTTTAA7980.22350249129656652No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTNGGAACACCTTGTTCA7850.21986147326792574No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTGGGTATGA7520.210618889041376No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGTATGA7440.20837826256221242No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNNGGTGGGAACACCTTGTTCA7190.20137630481482624No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTNGGGTGGGAACACCTTGTTCA7120.1994157566455581No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNGGGTGGGAACACCTTGTTCA6940.19437434706744006No Hit
TGCTTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACCTTGTTCA6940.19437434706744006No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGGTTTTA6930.19409426875754462No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATTTGCTTTAA6890.19297395551796281No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA6860.1921337205882765No Hit
GTCTCTCAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATCAGGCCTGA6830.19129348565859014No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATAGGGCTGGA6820.1910134073486947No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATTTGCTTTAA6820.1910134073486947No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATTTGGTTTTA6580.18429152791120398No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATTTGGTTTTA6420.17981027495287683No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATTTGCTTTAA6400.17925011833308593No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATTTGCTTTAA6380.17868996171329504No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATTTGCTTTAA6290.17616925692423602No Hit
CGGATGGTTNNGNACAACTTATNGNAAAGNTAAAGGAAACCCCAACATGC5970.16720675100758173No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTGGAACTCA5800.16244541973935914No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATTTGCTTTAA5800.16244541973935914No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGTTGCA5670.1588044017107183No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATCAGGTAAAA5640.15796416678103198No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATCAGGTAAAA5630.15768408847113652No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATTTGGTTTTA5590.15656377523155474No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATTTGGTTTTA5580.1562836969216593No Hit
TGCTTCTGANNGNTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA5460.15292275720291393No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATCAGGTAAAA5400.15124228734354125No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCTGGCTTTA5330.14928173917427315No Hit
GTCTCTCAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGCTTTAA5290.14816142593469134No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATCGAGCGTGA5280.14788134762479588No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTGAGTTCCA5240.1467610343852141No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATTTGGTTTTA5140.14396025128625964No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATTTGGTTTTA5140.14396025128625964No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATCAGGTAAAA5110.1431200163565733No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATCAGGTAAAA5100.14283993804667786No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATCGAGCGTGA5080.14227978142688696No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATCGAGCGTGA4940.13835868508835072No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATCTGGTTTTA4860.13611805860918713No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATCAGGTAAAA4850.13583798029929167No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATCAGGTAAAA4830.13527782367950078No Hit
GTCNCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCAGGCCTGA4810.1347176670597099No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATCGAGCGTGA4740.13275711889044178No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATCGAGCGTGA4710.1319168839607554No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGCTTCA4670.13079657072117362No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNGTTCTGGATATCAGGCCTGA4660.1305164924112782No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATCGAGCGTGA4590.12855594424201006No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTGGGTTTCA4590.12855594424201006No Hit
GTCTCTNAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATCAGGCCTGA4540.12715555269253284No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATCGAGCGTGA4520.12659539607274195No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATCTGGTTTTA4480.12547508283316014No Hit
GTCTCTCAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGGTTTTA4470.1251950045232647No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATACGGATGAA4470.1251950045232647No Hit
GTCTNTNAGNNGNTACACGGCAGNNTNAGNGTTCNGGATATCAGGCCTGA4460.12491492621336925No Hit
GTCTNTNAGNNGNTACACGGCAGNNTNAGNGTTCTGGATATCAGGCCTGA4420.12379461297378747No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNNTTCTGGATATCAGGCCTGA4360.12211414311441479No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATCTGGTTTTA4300.1204336732550421No Hit
GTCTNTNAGNNGGTACACGGCAGNGTNAGGGTTCTGGATATCAGGCCTGA4290.12015359494514666No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGGAATGA4240.11875320339566943No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATCTGGTTTTA4230.11847312508577398No Hit
GTCTCTCAGNNGNTACACGGCANGGTCAGNGTTCTGGATATCAGGCCTGA4200.11763289015608765No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATTTGGTTTAA4190.1173528118461922No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA4180.11707273353629676No Hit
GTCTCTCAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATCAGGTAAAA4100.11483210705713316No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGGTTTAA4090.11455202874723772No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATCTGGTTTTA4090.11455202874723772No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATTTGGTTTAA4090.11455202874723772No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGGGTTCTGGATATCAGGCCTGA4060.11371179381755138No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATCAGGCCTGA3990.11175124564828326No Hit
GTCNCTCAGNNGNTACACGGCANGNTCAGNNTTCTGGATATCAGGCCTGA3990.11175124564828326No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATGGGGTTTGA3990.11175124564828326No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATCTGGTTTTA3980.11147116733838781No Hit
GTCTCTNAGNNGGTACACGGCAGNNTNAGGGTTCTGGATATCAGGCCTGA3940.11035085409880603No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATTGGGTTTCA3930.11007077578891059No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATCTGGTTTTA3920.10979069747901514No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATTTGGTTTAA3900.10923054085922423No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATACGGATGAA3890.10895046254932879No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTNGGAACACCTTGTTCA3880.10867038423943334No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATACGGATGAA3860.10811022761964245No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATTGGGTTTCA3860.10811022761964245No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTGGGAACACCTTGTTCA3850.107830149309747No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGNGTTCNGGATATCAGGCCTGA3840.10755007099985156No Hit
GTCTCTCAGNNGNTACACGGCAGGNTCAGNGTTCTGGATATCAGGCCTGA3840.10755007099985156No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATTGGGTTTCA3830.10726999268995611No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATTTGGAATGA3810.10670983607016522No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATACGGATGAA3780.10586960114047889No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATACGGATGAA3780.10586960114047889No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATACGGATGAA3760.105309444520688No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATACGGATGAA3760.105309444520688No Hit
TGCTTCNGANNGNTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA3730.10446920959100166No Hit
GTCTCTNAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATTTGCTTTAA3720.10418913128110621No Hit
GTCTCTNAGNNGNTACACGGCANNNTNAGGGTTCNGGATATCAGGCCTGA3710.10390905297121074No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATGGGGTTTGA3700.10362897466131529No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATGGGGTTTGA3680.1030688180415244No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATGGGGTTTGA3670.10278873973162896No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATTGGGTTTCA3650.10222858311183806No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCAGGCCAGA3650.10222858311183806No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGCTAAAA3640.10194850480194262No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGCAATCA3630.10166842649204717No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATTTGGAATGA3620.10138834818215173No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTNGGGTNGGAACACCTTGTTCA3610.10110826987225628No Hit
GTCTNTNAGNNGNTACACGGCAGNNTNAGNGTTCTGGATATTTGCTTTAA3610.10110826987225628No Hit
TGCTNCNGANNGCTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA3610.10110826987225628No Hit
GTCTNTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATCAGGCCTGA3600.10082819156236084No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGGTATGA3590.10054811325246539No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)