FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004953604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112992
Sequences flagged as poor quality0
Sequence length35-300
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGCGCCCGAATCTGGCTTGGCGGAATATCTCTTTGACAAGCACACCC2590222.92374681393373No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN98588.724511469838573No Hit
GGGACGCGGCTCCTGGTGCTCGAGGACCTGAAAAACGTGTTCCCACCCGA46444.1100254885301615No Hit
GGGGTGACTTCCCTGGTCACCAAGGCAGTGCATGCATGTTGGGGTTTCCT44083.901161144151798No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG37703.3365193996035116No Hit
GGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACGA20591.8222529028603793No Hit
GGGAAACAGAATGGCTTTTTGGCTGAGAAGGCTGGGTCTACATTTCAGGC13901.2301755876522231No Hit
GGGGATGCTTGCATGTGTAATCTTACTAAGAGCTAATAGAAAGGCTAGGACCAAACCATCCG13301.1770744831492495No Hit
GGGAGAGCGGGCTGAATGCAATGGAGTGTGCATTACATTTGGAAAAAAAT11401.0089209855564996No Hit
GGGACTGAGAAGGCTCACGAAGGGCATCCGCAATGTTGGTTTCACTGAGA9460.837227414330218No Hit
GGGGAGGCCAGTGTTGTGGGCTTCCTGCTGGGACTGAGAAGGCTCACGAA8320.7363353157745681No Hit
GGGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACG7960.7044746530727839No Hit
GGGGAGGAGACTTTTCACTCTGCAGGGGAGCGCTGTCAGCATGACACGTG5940.5257009345794392No Hit
GGGAGGAAGAAAAGGAAAGTGAAGAAGCCATCCTAGCAGATGATGATGAA5340.47259983007646555No Hit
GGGAGACCTCCCGGCTCGACAGGCGGCGCGGGCGGCGGTAAAATGTCGGT5050.44693429623336167No Hit
GGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG4500.3982582837723025No Hit
GGGGAAGCAAGACGTCCTGGAATGAAGCATGTAGCTCTATGGGGGGAGGGGGGAGGGCTGCCTGAAAACCATCCG4500.3982582837723025No Hit
GGGGATCTGGCACTGTGGTTCCTGCATGAAGACAGTGGCTGGCGGTGCCT4350.3849830076465591No Hit
GGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACAGTGC3900.34515717926932876No Hit
GGGGATCACAGCTGCCGTGCATGACCTCTCTGCCACCTACTTCTGTGCCG3460.30621636930048146No Hit
GGGGTTTCCTTTACCTTTTCTATAAGTTGTACCAAACCATCCG3450.30533135089209856No Hit
GGGGGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGC3170.2805508354573775No Hit
GGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACAT3090.2734706881903144No Hit
GGGAGGCTGACCGTACTGGGTAAGGAGGCGGTTGGGGCTCCGGAGAGCTC3030.268160577740017No Hit
GGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTT2850.2522302463891249No Hit
GGGAAAAACATTGAAGACGTCATTGCCCAGGGTATTGGCAAGCTTGCCAGTGTACCAGCTGAGAGAC2640.23364485981308408No Hit
GGGACGGCTCACCGTGCTAGAGGACCTGAAAAACGTGTTCCCACCCGAGG2420.2141744548286604No Hit
GGGGGTAATTCTACTGGGTTTTGCATCCAGACTGAGTTTCCTTCCCTCAC2240.19824412347776835No Hit
GGGAAGCGAAAGGCCAAGGACCCCCCTCGGTCTCCGCCGGATTCTGAGTC2170.19204899461908806No Hit
GGGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGT2110.1867388841687907No Hit
GGGAATGTGCACTTGAACAATGAAGTTGGTGACAAGCATTACTGTACTCC1900.16815349759274995No Hit
GGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTG1850.16372840555083545No Hit
GGGGAGAACAGCGTCTTTTACCTGTCACCGAAGCCAGGAAGCCCCGTTTG1820.16107335032568676No Hit
GGGGGACAAGGCACCAGACTCACAGTTGTAGAGGACCTGAACAAGGTGTT1810.1601883319173039No Hit
GGGAGACAGCTCTCAGAGCAACCCTAGCCCCATTACCTCTTCCCTTTCCA1740.15399320305862363No Hit
GGGGCATTGCGGGTCCGGCACTCAAGTGATTGTTTTACCACATATCCAGA1570.13894789011611441No Hit
GGGGTAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTT1570.13894789011611441No Hit
GGGGAAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTG1560.13806287170773152No Hit
GGGGATGAAAAGGATGTGTGGGCTTCTGGGTGTTTTTGACTGACTAAGAA1550.13717785329934862No Hit
GGGACTGAAGCTTTCTTTGGACAAGGCACCAGACTCACAGTTGTAGAGGA1510.13363777966581705No Hit
GGGAAAAACATCCTGAGGACAGTGCCTGGAGATACGCAGTATTTTGGCCC1410.1247875955819881No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT1410.1247875955819881No Hit
GGGACTCATGATGCACTGTGTAGCAATCAGCCCCAGCATTTTGGTGATGG1390.12301755876522232No Hit
GGGAGGAGGAGGTGCTGACGGACTCACCTTTGGCAAAGGGACTCATCTAA1210.1070872274143302No Hit
GGGAGATTTGCAGCTTTCTAGGCAGGAGACAAGACAATCTGCATCTTCAC1170.10354715378079864No Hit
GGGAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAACAAGGTGTTCC1130.10000708014726706No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)