FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004953606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70602
Sequences flagged as poor quality0
Sequence length35-300
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGCGCCCGAATCTGGCTTGGCGGAATATCTCTTTGACAAGCACACCC2382933.75116852213818No Hit
GGGGTGACTTCCCTGGTCACCAAGGCAGTGCATGCATGTTGGGGTTTCCT30624.336987620747287No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14652.0750120393189997No Hit
GGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACGA14172.007025296733804No Hit
GGGAGAGCGGGCTGAATGCAATGGAGTGTGCATTACATTTGGAAAAAAAT13321.8866321067391858No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG12111.7152488598056712No Hit
GGGACTGAGAAGGCTCACGAAGGGCATCCGCAATGTTGGTTTCACTGAGA7301.0339650434831875No Hit
GGGAGATCAGAAGAGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGCA5230.7407722160845301No Hit
GGGGAGGCCAGTGTTGTGGGCTTCCTGCTGGGACTGAGAAGGCTCACGAA5150.7294410923203308No Hit
GGGAGACCTCCCGGCTCGACAGGCGGCGCGGGCGGCGGTAAAATGTCGGT5080.7195263590266564No Hit
GGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG4400.6232118070309622No Hit
GGGGATGCTTGCATGTGTAATCTTACTAAGAGCTAATAGAAAGGCTAGGACCAAACCATCCG2810.39800572221750086No Hit
GGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTT2530.3583467890428033No Hit
GGGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACG2470.34984844621965383No Hit
GGGAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTTCC2370.3356845415144047No Hit
GGGGTTTCCTTTACCTTTTCTATAAGTTGTACCAAACCATCCG1920.2719469703407835No Hit
GGGGGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGC1910.27053057987025864No Hit
GGGGAGAACAGCGTCTTTTACCTGTCACCGAAGCCAGGAAGCCCCGTTTG1830.2591994561060593No Hit
GGGGAGATCAGAAGAGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGC1820.2577830656355344No Hit
GGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACAT1800.25495028469448455No Hit
GGGGATCTGGCACTGTGGTTCCTGCATGAAGACAGTGGCTGGCGGTGCCT1690.2393699895187105No Hit
GGGGAAGCAAGACGTCCTGGAATGAAGCATGTAGCTCTATGGGGGGAGGGGGGAGGGCTGCCTGAAAACCATCCG1630.230871646695561No Hit
GGGACAAAGACATCCAGCTCTAAGGAGCTCAAAACATCCTGAGGACAGTG1620.22945525622503612No Hit
GGGAAAACAGCCTTACAAAGACATCCAGCTCTAAGGAGCTCAAAACATCC1600.22662247528398627No Hit
GGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACAGTGC1580.22378969434293647No Hit
GGGGAGTTGCTGCTGGGCTCATTGCAGCTCAGACACAGCAAAAGAGCCTA1580.22378969434293647No Hit
GGGATTGCAGACACCCAGACTGGGGACTCAGCTATCTACTTCTGTGCAGA1560.22095691340188664No Hit
GGGAGGAAGAAAAGGAAAGTGAAGAAGCCATCCTAGCAGATGATGATGAA1560.22095691340188664No Hit
GGGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGT1470.20820939916716238No Hit
GGGAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTTC1410.1997110563440129No Hit
GGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTAT1400.198294665873488No Hit
GGGAGAAAGACTAGGGATTCACCCAGTAAAGAGAGCTCATCTGTGACTGA1300.18413076116823887No Hit
GGGAAGCGAAAGGCCAAGGACCCCCCTCGGTCTCCGCCGGATTCTGAGTC1270.1798815897566641No Hit
GGGAGAAACACAGTGGAAGATTAAGAGTCACGCTTGACACTTCCAAGAAA1250.1770488088156143No Hit
GGGAGTGATTCAGCCACCTACCTCTGTGTGGTGAACACGCTCTCCGGCAA1190.16855046599246482No Hit
GGGTGACCACGTGACCAACTTGCGCAAGATGGGAGCGCCCGAATCTGGCT1150.16288490411036513No Hit
GGGGGCACTGCCAGTAAACTCACCTTTGGGACTGGAACAAGACTTCAGGT1150.16288490411036513No Hit
GGGAACAAACTGGTCTTTGGCGCAGGAACCATTCTGAGAGTCAAGTCCTA1100.15580295175774056No Hit
GGGAGGAAGCTACATACCTACATTTGGAAGAGGAACCAGCCTTATTGTTC1070.15155378034616582No Hit
GGGCAGACAGACTTGTCACTGAATTTAACAAGAGTCAAACTTCCTTCCAC1060.15013738987564093No Hit
GGGAGAGACTCCCAGCCCAGTGATTCAGCCACCTACCTCTGTGCCGTGAA1020.14447182799354125No Hit
GGGGGACTCAGCAATGTATTTCTGTGCAATGAGAGAGTATGAAGGTAACC1000.14163904705249145No Hit
GGGAGTCCATATAAGCGACACGGCTGAGTACTTCTGTGCTGTGACTTTAT990.1402226565819665No Hit
GGGGGACGAAACCCTCAGCCCATATGAGCGACGCGGCTGAGTACTTCTGT960.13597348517039176No Hit
GGGGGACTCAGCAATGTATTTCTGTGCAATGAGAGAGGCAAGAGGCTCAA930.13172431375881702No Hit
GGGAATGTGCACTTGAACAATGAAGTTGGTGACAAGCATTACTGTACTCC930.13172431375881702No Hit
GGGAAAAACATTGAAGACGTCATTGCCCAGGGTATTGGCAAGCTTGCCAGTGTACCAGCTGAGAGAC920.13030792328829213No Hit
GGGATTCAGGAGGAGGTGCTGACGGACTCACCTTTGGCAAAGGGACTCAT910.1288915328177672No Hit
GGGGATTCAGGAGGAGGTGCTGACGGACTCACCTTTGGCAAAGGGACTCA890.1260587518767174No Hit
GGGGACAATGAGCAGTTCTTCGGGCCAGGGACACGGCTCACCGTGCTAGA880.12464236140619245No Hit
GGGGAAAACAGCCTTACAAAGACATCCAGCTCTAAGGAGCTCAAAACATC880.12464236140619245No Hit
GGGGAATGTGCACTTGAACAATGAAGTTGGTGACAAGCATTACTGTACTC870.12322597093566755No Hit
GGGGATGCTCAACCACTGTTTTTGTAGAGGAGTTTGACGCTGTGTGGAAT860.12180958046514262No Hit
GGGAGGGAGACAGCGCTGTTATCAAGTGTACTTATTCAGACAGTGCCTCA850.12039318999461772No Hit
GGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAACAAGGTGTT840.1189767995240928No Hit
GGGAGCGCCCGAATCTGGCTTGGCGGAATATCTCTTGACAAGCACACCCT830.11756040905356788No Hit
GGGAGTGATTCAGCCACCTACCTCTGTGTGGTGAACGAGGACCCATCAGG820.11614401858304298No Hit
GGGAGACAGTGATAATGAAAGCTAAGCCTCGGGCTAATTTCCCCATAGCC810.11472762811251806No Hit
GGGACACTAAAGATGAACTATTCTCCAGGCTTAGTATCTCTGATACTCTT810.11472762811251806No Hit
GGGAGTGCTTTGCCAACCTGGCCTGTTTGATCTGGTTTTTGTTGTTGAGC800.11331123764199313No Hit
GGGAGGCAGGAAGCCGGACCTCGGGGGACGCAGCAGATATCCAGAACCCT790.11189484717146823No Hit
GGGGAGAATGTGGAGCAGCATCCTTCAACCCTGAGTGTCCAGGAGGGAGA790.11189484717146823No Hit
GGGGATATCAGCCCCAGCATTTTGGTGATGGGACTCGACTCTCCATCCTA780.11047845670094332No Hit
GGGCAAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTG760.10764567575989349No Hit
GGGGAGCATCTCTGGGGTTTTTGCAAAGAAAGGAAACTCTGTGCATACTC750.10622928528936856No Hit
GGGGAGGTGTAACCTCTGGCTGTCTCTTCTGCCTGGTTTTTGTTGAGCTT740.10481289481884366No Hit
GGGAGAAACCATTTGCTCAAGAGGAAGACTCAGCCATGTATTACTGTGCT720.10198011387779382No Hit
GGGGATGATAGAAGTAACTCTTATAACTGGAGGTTGCAGGTCAATGACTG710.10056372340726892No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)