FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182620055
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG2263430.12394202816333617TruSeq Adapter, Index 7 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA608700.061.1287961
TCGGAAG673550.055.4587482
CGGAAGA760650.048.962183
AGAGCAC1811450.021.0944617
AGCACAC1937300.019.7054279
GAGCACA1988600.019.1825058
GAAGAGC2047550.018.8852025
TATGCCG426000.018.12429445-49
CTCGTAT459150.016.28806340-44
TCGTATC473550.016.11822535-39
TCTCGTA472950.015.82812440-44
CGTATGC488750.015.65793345-49
TATCTCG493150.014.85055940-44
GTCACGA521550.014.77872325-29
ATGCCGT537800.014.51288745-49
TGCCGTC544650.013.90183445-49
GCCGTCT561750.013.66673450-54
TCGTATG537700.013.66328140-44
ATTCGTA560400.013.58144135-39
ATCTCGT567650.013.22840840-44
Produced by FastQC (version 0.11.5)