FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182620055
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTC2156900.1181086053226739Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG699600.055.5604939
GAGCGTC844600.046.1003158
ATCGGAA929450.043.469931
TCGGAAG990700.040.3306472
AGAGCGT971700.040.2991757
CGGAAGA1068600.037.33533
AAGAGCG1154950.034.369656
GAAGAGC2357350.017.3740735
CGCCGTA495200.016.0961155-59
TCGCCGT544900.014.62001255-59
GCCGTAT592050.013.663836555-59
GTCGCCG609000.013.13594255-59
TGGTCGC641200.012.64133350-54
CCGTATC639900.012.40420255-59
CGTATCA666600.011.99509460-64
GTGGTCG683350.011.95070350-54
GGTCGCC675500.011.8127250-54
GCGTCGT678150.011.75650610-14
CGTCGTG691850.011.53208810-14
GGAAGAG3823650.011.0447294
Produced by FastQC (version 0.11.5)