FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460004742
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATG25363690.5513788812203158TruSeq Adapter, Index 1 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA4884850.086.219681
TCGGAAG5007600.084.06542
CGGAAGA5189100.081.013893
AGAGCAC7679250.054.908297
AGCACAC7904600.053.2753949
GAGCACA8061200.052.3273478
GAAGAGC8314400.050.8864445
AAGAGCA10916000.038.97986
GGAAGAG12057750.035.4004144
TATGCCG3438900.024.8476145-49
CTCGTAT3370400.023.7774940-44
CGTATGC3574300.023.69908345-49
TCTCGTA3425750.023.36240-44
ATGCCGT3700250.023.04128845-49
AGCGATA3699100.023.04039230-34
CGATAGA3680900.022.93231235-39
GCGATAG3682850.022.76096730-34
TGCCGTC3694000.022.67486245-49
GCCGTCT3726250.022.5854250-54
TCGTATG3517700.022.39525440-44
Produced by FastQC (version 0.11.5)