FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460004742
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC27524840.59835991864623Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG4709300.090.184989
GAGCGTC5074100.083.74448
ATCGGAA5535150.078.053581
AGAGCGT5474900.077.77797
TCGGAAG5647250.076.173482
CGGAAGA5820600.073.709673
AAGAGCG5933650.072.184946
GAAGAGC8918000.048.396135
GGAAGAG12639100.034.446894
CGCCGTA3415700.025.07581555-59
TCGCCGT3543650.024.16549755-59
GCCGTAT3667450.023.41500555-59
GTCGCCG3684000.023.18738455-59
TGGTCGC3818650.022.56247950-54
CCGTATC3785600.022.31873555-59
CGTATCA3868600.022.04639660-64
GGTCGCC3852350.022.01758650-54
GTGGTCG4003300.021.60295550-54
GTACGTC4004650.021.46261630-34
TACGTCC4095700.021.10976630-34
Produced by FastQC (version 0.11.5)