FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457842520
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCTC9076530.1982456762644064Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2291450.062.4777649
GAGCGTC2682100.053.4886448
ATCGGAA2971100.049.3784831
TCGGAAG3108350.047.0888372
AGAGCGT3058350.047.0755427
CGGAAGA3345000.043.6665043
AAGAGCG3495900.041.6665656
GAAGAGC6439300.023.1588925
CGCCGTA1440400.020.28409655-59
TCGCCGT1567550.018.63507755-59
GCCGTAT1684050.017.4492255-59
GTCGCCG1707650.017.23608255-59
TGGTCGC1809600.016.35910450-54
CCGTATC1806250.015.88430455-59
CGTATCA1892050.015.5334460-64
GGTCGCC1883250.015.40607250-54
GTGGTCG1945350.015.33675550-54
GGAAGAG10108350.015.1345324
CAGTACG2005750.014.38663530-34
AGTACGT2124300.013.98125435-39
Produced by FastQC (version 0.11.5)