FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91572947
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC1818910.1986296236594854Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG547450.071.163739
GAGCGTC622200.062.7307558
ATCGGAA687200.057.8889771
AGAGCGT692800.056.526577
TCGGAAG712250.055.7044752
CGGAAGA764500.051.8552863
AAGAGCG788750.049.9966776
GAAGAGC1380450.028.9868265
CGCCGTA373050.021.6051455-59
TCGCCGT401100.020.09423655-59
GGAAGAG2130100.019.2555924
GCCGTAT421600.019.23409355-59
GTCGCCG430250.018.70586855-59
TGGTCGC450050.018.08868850-54
CCGTATC445000.017.71767455-59
CGTATCA458850.017.49544760-64
GGTCGCC461350.017.23395550-54
GTGGTCG474400.017.22746750-54
CGTCGTG533250.015.11696310-14
TCTCGGT545950.015.06560245-49
Produced by FastQC (version 0.11.5)