FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442091363
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG29044690.6569838823112226TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA6049950.079.555051
TCGGAAG6154900.078.328412
CGGAAGA6349600.075.852513
AGAGCAC8530850.056.4431277
AGCACAC8748550.054.9010359
GAGCACA8899400.054.104038
GAAGAGC9226850.052.423575
AAGAGCA11673950.041.569886
GGAAGAG12699150.038.4332164
TATGCCG4183850.023.441345-49
CTCGTAT4177100.022.50678840-44
CGTATGC4319750.022.33730145-49
TCTCGTA4230450.022.20890440-44
ATGCCGT4451300.022.05045345-49
TGCCGTC4459700.021.6366645-49
GCCGTCT4469350.021.51014550-54
TCGTATG4305550.021.49689940-44
TATCTCG4327350.021.39329540-44
CGCTCAT4635700.020.96864130-34
ATCTCGT4488600.020.914540-44
Produced by FastQC (version 0.11.5)