FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442091363
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC30699270.6944100828316793Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG5546950.086.522819
GAGCGTC5939700.080.80058
AGAGCGT6305100.076.276067
ATCGGAA6454400.075.301681
TCGGAAG6543900.074.102992
CGGAAGA6762750.071.661243
AAGAGCG6833400.070.677166
GAAGAGC9649200.050.3812985
GGAAGAG13271300.037.058284
CGCCGTA3974400.024.46394355-59
TCGCCGT4125050.023.57085455-59
GCCGTAT4187900.023.28566655-59
GTCGCCG4295100.022.54548855-59
TGGTCGC4372750.022.39197350-54
CCGTATC4290100.022.37033555-59
CGTATCA4365200.022.14670860-64
GGTCGCC4422500.021.81977350-54
GTGGTCG4530250.021.68805950-54
TCTCGGT4860050.020.2766945-49
TCGGTGG4930950.019.72079745-49
Produced by FastQC (version 0.11.5)