FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323162695
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG10151290.3141232003898222TruSeq Adapter, Index 7 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2765850.060.7417141
TCGGAAG2836750.059.015512
CGGAAGA2963950.056.4789623
AGAGCAC4685150.035.945167
AGCACAC4857800.034.725849
GAGCACA4971450.033.892628
GAAGAGC5112150.033.0704155
AAGAGCA7002200.024.4245346
GGAAGAG7734800.022.2245754
TATGCCG1668100.020.7766145-49
CGTATGC1762650.019.45895845-49
CTCGTAT1701850.019.3544240-44
TCTCGTA1743150.018.8858840-44
TCGTATC1841400.018.72283635-39
ATGCCGT1878500.018.51900945-49
TGCCGTC1859450.018.12235545-49
TATCTCG1798750.018.05222540-44
TCGTATG1790850.017.87844340-44
GCCGTCT1915750.017.76846750-54
ATCTCGT1914850.017.16441340-44
Produced by FastQC (version 0.11.5)