FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323162695
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC10682490.330560741239022Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2421500.068.9105459
GAGCGTC2672950.062.4768268
AGAGCGT2950450.056.8717967
ATCGGAA3013000.056.1913381
TCGGAAG3082700.054.87672
CGGAAGA3227450.052.339313
AAGAGCG3288150.051.240386
GAAGAGC5370850.031.826655
CGCCGTA1532100.022.35370355-59
GGAAGAG7987450.021.7937874
TCGCCGT1630450.020.99019455-59
GCCGTAT1708350.020.15186755-59
GTCGCCG1704800.019.9659155-59
TGGTCGC1788350.019.2893950-54
CCGTATC1785350.018.71428755-59
GGTCGCC1811450.018.55998250-54
CGTATCA1836250.018.53187460-64
GTGGTCG1882500.018.38165950-54
TCTCGGT2141400.016.3555845-49
TCGGTGG2163350.015.74663745-49
Produced by FastQC (version 0.11.5)