FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444051204
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG28183280.6346853638978085TruSeq Adapter, Index 7 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA6795800.066.401341
TCGGAAG6859350.065.875982
CGGAAGA7086000.063.766853
AGAGCAC9148350.049.5251247
AGCACAC9322100.048.519059
GAGCACA9459550.047.8789948
GAAGAGC9927250.045.781455
AAGAGCA12296550.037.116856
GGAAGAG13364200.034.2793464
TATGCCG4367650.021.12163245-49
CTCGTAT4352400.020.3301940-44
CGTATGC4509750.020.27544445-49
TCTCGTA4420100.019.98043440-44
ATGCCGT4692150.019.67511245-49
TATCTCG4506850.019.43630640-44
TCGTATC4741650.019.42473835-39
TCGTATG4500200.019.27207840-44
TGCCGTC4690800.019.23698845-49
CGTCTTC4852500.019.02517950-54
GCCGTCT4825050.018.95699950-54
Produced by FastQC (version 0.11.5)