FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400204418
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG55085981.3764460741160534TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA10229500.088.73621
TCGGAAG10278900.088.3276752
CGGAAGA10576400.085.8086243
AGAGCAC12151400.074.46447
AGCACAC12271300.073.42029
GAGCACA12404000.072.8848658
GAAGAGC13014850.069.700615
AAGAGCA15017700.060.484196
GGAAGAG15866600.057.504084
TATGCCG7725700.023.60359645-49
CTCGTAT7635950.023.0839540-44
CGTATGC7880300.022.85277445-49
TCTCGTA7710750.022.8310640-44
ATGCCGT8066200.022.62105445-49
TCGTATG7779600.022.34293740-44
TATCTCG7804750.022.26611140-44
TGCCGTC8116900.022.19574445-49
CCGTCTT8213950.022.19077950-54
CGTCTTC8256800.022.11389550-54
ATCTCGT7962350.022.09998140-44
Produced by FastQC (version 0.11.5)