FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400204418
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCTC58479791.4612479865227275Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG9796100.092.189579
GAGCGTC10164200.088.829518
AGAGCGT10483850.086.207457
ATCGGAA10690900.085.050291
TCGGAAG10720500.084.6765442
AAGAGCG10969200.082.581576
CGGAAGA10990700.082.531283
GAAGAGC13386700.067.859595
GGAAGAG16166100.056.4080544
CGCCGTA7541150.024.08573255-59
GCCGTAT7716900.023.60350655-59
TCGCCGT7824000.023.18590255-59
CCGTATC7786750.023.07841155-59
CGTATCA7827400.023.03293460-64
TGGTCGC7960000.022.76220150-54
GTCGCCG8007400.022.46419755-59
GGTCGCC8023100.022.32783750-54
GTGGTCG8163400.022.21725750-54
TCTCGGT8424800.021.73326945-49
CTCGGTG8620950.021.21388645-49
Produced by FastQC (version 0.11.5)