FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953764

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953764
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100870883
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA394950.047.344691
TCGGAAG424650.043.9507062
CGGAAGA478500.038.9893723
CGCGAAA186700.019.94037835-39
TCCGCGA192150.019.57847230-34
CCGCGAA196050.018.78245430-34
AGAGCAC1024600.018.7046727
AGCACAC1111000.017.2958419
TATGCCG225300.017.20031445-49
GAGCACA1130450.017.0110768
GAAGAGC1172000.016.4078665
CTCCGCG245650.015.60944930-34
CGTATGC260850.014.71171545-49
GCGAAAT261650.014.57735735-39
CTCGTAT227850.014.46401640-44
TCTCGTA237700.013.80977140-44
ATGCCGT285250.013.73271945-49
ACTCCGC282600.013.6197930-34
TGCCGTC285200.013.31336845-49
GCCGTCT299850.012.6916850-54
Produced by FastQC (version 0.11.5)