FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences439651443
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG10694740.24325497323569573TruSeq Adapter, Index 13 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA3657800.047.209041
TCGGAAG3787700.045.703282
CGGAAGA3924600.044.1005063
AGAGCAC6253400.028.0205527
AGCACAC6471900.027.1093929
GAGCACA6611950.026.5614938
GAAGAGC6886700.025.5143135
AAGAGCA9324950.019.1290236
TATGCCG1887650.018.84528245-49
GGAAGAG10371300.017.2960224
CGTATGC2043350.017.18933545-49
CTCGTAT2004000.017.1415540-44
TCTCGTA2054800.016.74599340-44
ATGCCGT2158850.016.56251345-49
TGCCGTC2139350.016.35834945-49
GCCGTCT2168400.016.2389150-54
TCGTATG2149900.015.69500840-44
ATCTCGT2295200.015.02991540-44
CGTCTTC2439400.014.7582250-54
CCGTCTT2470450.014.51990950-54
Produced by FastQC (version 0.11.5)