FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416089548
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG15162910.3644145851027241TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA4909850.050.078891
TCGGAAG4978150.049.4900362
CGGAAGA5116000.048.1182443
AGAGCAC7224050.034.3039787
AGCACAC7379750.033.482119
GAGCACA7541250.032.8525478
GAAGAGC7863550.031.5651325
AAGAGCA10202150.024.5951756
GGAAGAG11257400.022.3517274
TATGCCG2442300.020.57704545-49
CGTATGC2598300.019.09664345-49
CTCGTAT2545950.018.99851240-44
ATGCCGT2698800.018.7024845-49
TCTCGTA2603800.018.56193540-44
TGCCGTC2665400.018.55442645-49
GCCGTCT2703200.018.33575250-54
TCGTATG2654900.017.93487740-44
CGTCTTC2919800.017.31322350-54
ATCTCGT2841650.017.05725940-44
CCGTCTT2966400.017.0109450-54
Produced by FastQC (version 0.11.5)