FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472938772
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATG13019330.27528574037063724TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA4042050.051.9104271
TCGGAAG4135100.050.6275442
CGGAAGA4376500.047.8453983
AGAGCAC6791400.031.058837
AGCACAC7010550.030.0972549
GAGCACA7206400.029.3245628
GAAGAGC7521550.028.2134425
AAGAGCA10111900.021.1918016
GGAAGAG11216250.019.2038694
TATGCCG2285900.018.7075445-49
CTCGTAT2366550.017.55993740-44
CGTATGC2432750.017.4209445-49
TCTCGTA2434100.017.05832540-44
ATGCCGT2611050.016.4889845-49
CGGCTAT2611550.016.27492130-34
TCGTATG2518200.016.22608240-44
TGCCGTC2632200.016.02984445-49
GCCGTCT2698400.015.75501950-54
ATCTCGT2717300.015.33811240-44
ACCGGCT2864100.014.86211130-34
Produced by FastQC (version 0.11.5)