FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472613326
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG10702500.22645362310414413TruSeq Adapter, Index 27 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA3302200.052.8967931
TCGGAAG3426250.051.0603942
CGGAAGA3661000.047.7786943
AGAGCAC6066000.029.1847867
AGCACAC6392300.027.7460569
GAGCACA6522600.027.1528848
GAAGAGC6811750.026.1088285
AAGAGCA9361350.019.2628146
TATGCCG1903200.018.76172645-49
CGTATGC2043100.017.35284645-49
CTCGTAT1981600.017.33783740-44
GGAAGAG10526650.017.241394
TCTCGTA2045500.016.7997640-44
ATGCCGT2207200.016.27749445-49
TTACTCG2206200.015.92318530-34
TGCCGTC2233450.015.86028645-49
GCCGTCT2239700.015.81620350-54
TCGTATG2155950.015.77098440-44
TACTCGA2273950.015.53712535-39
ATCTCGT2305550.014.96963640-44
Produced by FastQC (version 0.11.5)