FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428424015
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATG7806270.1822089735095732TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA3931250.032.7796481
TCGGAAG4013150.032.3345642
CGGAAGA4179600.031.0399173
AGAGCAC6368800.020.647427
AGCACAC6540950.020.0309039
GAGCACA6718000.019.6432138
GAAGAGC7028650.018.8698885
TATGCCG1781000.014.8409945-49
AAGAGCA9496100.014.2346426
CGTATGC1931100.013.59734145-49
CTCGTAT1901400.013.46781940-44
TCTCGTA1968600.013.02354840-44
GGAAGAG10472350.012.969624
ATGCCGT2063650.012.90944645-49
TGCCGTC2053050.012.69927445-49
TCGTATG2008300.012.54881840-44
GCCGTCT2132200.012.37133750-54
CGTCTTC2287900.011.73284950-54
ATCTCGT2216300.011.63798840-44
CCGTCTT2314200.011.57444850-54
Produced by FastQC (version 0.11.5)