FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438956664
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC33139200.7549537965324067Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG7226750.070.4426969
GAGCGTC7597300.067.014568
AGAGCGT7926800.064.3532647
TCGGAAG8246750.062.3143042
ATCGGAA8283650.062.0939331
AAGAGCG8579000.059.6510246
CGGAAGA8757800.058.6284523
GAAGAGC11295850.045.599475
GGAAGAG14042250.036.9620824
CGTATCA5118500.019.95456760-64
CGCCGTA5246950.019.72461555-59
GCCGTAT5322350.019.48361655-59
CCGTATC5284400.019.28339255-59
TGGTCGC5426450.019.16440650-54
GTGGTCG5598250.018.63977650-54
TCGCCGT5623350.018.42395255-59
TCTCGGT5666200.018.41000645-49
GGTCGCC5647600.018.1493750-54
TACGTCC5788500.017.86255530-34
GTACGTC5749250.017.82316430-34
Produced by FastQC (version 0.11.5)