FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454008438
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCTC10594390.23335227086682472Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2174500.077.436669
ATCGGAA2641700.065.4106451
GAGCGTC2591900.065.1647648
TCGGAAG2822000.061.0023962
AGAGCGT2873300.059.1736837
CGGAAGA3035550.056.5568283
AAGAGCG3273350.052.325856
GAAGAGC6142450.028.3772165
CGCCGTA1630950.020.82811755-59
TCGCCGT1809700.018.77245955-59
GGAAGAG9784550.018.2199654
GCCGTAT1884800.018.18831455-59
GTCGCCG1944350.017.48063355-59
CCGTATC2015500.016.80239755-59
TGGTCGC2094450.016.41354250-54
CGTATCA2088050.016.37405660-64
GGTCGCC2135750.015.87213550-54
GCGTCGT2178600.015.72325810-14
GTGGTCG2229200.015.463654550-54
CGTCGTG2236250.015.340610510-14
Produced by FastQC (version 0.11.5)