FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419986907
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC78612081.8717745408191975Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG10733450.0120.039639
ATCGGAA11349150.0116.566291
GAGCGTC11130350.0115.758
TCGGAAG11666900.0113.426922
AGAGCGT11465200.0112.4343957
CGGAAGA11941750.0110.885963
AAGAGCG12315400.0107.252646
GAAGAGC14968850.088.480355
GGAAGAG18822400.070.6749954
TCCGATC652500.034.913693
AGCGGCG1084250.032.0830549
TTCCGAT714500.032.0115932
CGCCGTA9285750.026.6222155-59
GCCGTAT9455200.026.14801655-59
TCGCCGT9242850.026.13909555-59
CCGATCT817850.025.9510884
CCGTATC9576100.025.84377955-59
CGTATCA9660300.025.5346460-64
GTCGCCG9717350.025.48915555-59
TGGTCGC9804700.025.3063250-54
Produced by FastQC (version 0.11.5)