FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394152177
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTATG37495940.9513061753303471TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA6771650.091.755481
TCGGAAG6853500.090.687462
CGGAAGA7056450.088.000663
AGAGCAC9000850.068.813377
AGCACAC9181200.067.304299
GAGCACA9304800.066.488398
GAAGAGC9595900.064.8604355
AAGAGCA11809400.052.681816
GGAAGAG12733200.049.1899954
TATGCCG5149750.024.40544745-49
TCCGCGA5223050.024.0113730-34
CGCGAAA5172100.023.95190635-39
CTCGTAT5019800.023.73547240-44
CCGCGAA5220200.023.59784530-34
CGTATGC5275850.023.555345-49
TCTCGTA5064350.023.50234240-44
ATGCCGT5376000.023.34247245-49
GCCGTCT5366900.023.08358850-54
GCGAAAT5437300.022.98818235-39
TGCCGTC5370200.022.96570245-49
Produced by FastQC (version 0.11.5)