FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394152177
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCTC39490861.0019191140989183Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG6341850.097.2308049
GAGCGTC6682100.092.255398
AGAGCGT6986100.088.372737
ATCGGAA7123350.087.581141
TCGGAAG7198600.086.523612
CGGAAGA7371250.084.4066543
AAGAGCG7377800.083.7356646
GAAGAGC9815150.063.196855
GGAAGAG12874700.048.7510834
CGCCGTA4937200.025.29769755-59
TCGCCGT5107950.024.46452755-59
GCCGTAT5148850.024.30145355-59
GTCGCCG5201600.023.91701955-59
TGGTCGC5327800.023.52902650-54
CCGTATC5257950.023.4436655-59
CGTATCA5316950.023.35480360-64
GGTCGCC5297200.023.2313950-54
GTGGTCG5493100.022.86057750-54
TCTCGGT5809850.021.80769345-49
CTCGGTG5901700.021.37174245-49
Produced by FastQC (version 0.11.5)