FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences439244613
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTC9262990.2108845441890485Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2042600.072.153369
GAGCGTC2407200.061.324168
ATCGGAA2590850.058.680711
TCGGAAG2759600.054.752862
AGAGCGT2777000.053.497087
CGGAAGA3024350.049.9102553
AAGAGCG3223400.046.585686
GAAGAGC5972350.025.7012565
CGCCGTA1496100.019.89559255-59
TCGCCGT1639550.018.20611855-59
GCCGTAT1748150.017.20440955-59
GTCGCCG1789600.016.69555-59
GGAAGAG9587550.016.421924
CCGTATC1869050.015.79383255-59
TGGTCGC1922500.015.76545250-54
CGTATCA1945200.015.40427860-64
GCGTCGT1962400.015.29883910-14
GGTCGCC1972550.015.10171250-54
CGTCGTG2014050.014.93887710-14
GTGGTCG2071100.014.73433950-54
Produced by FastQC (version 0.11.5)