FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448812856
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTC12820900.2856624944807731Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2997500.066.424239
GAGCGTC3392600.058.771658
ATCGGAA3748300.054.032021
AGAGCGT3756800.053.230377
TCGGAAG3891750.051.9643442
CGGAAGA4062400.049.707163
AAGAGCG4269750.047.233416
GAAGAGC7123750.028.738625
CGCCGTA1882200.021.43194455-59
TCGCCGT2024950.019.9203755-59
GGAAGAG10695550.019.5165884
GCCGTAT2124750.019.12738655-59
GTCGCCG2154900.018.66927155-59
CCGTATC2225850.017.96730655-59
TGGTCGC2274700.017.9136850-54
CGTATCA2305950.017.47666460-64
GGTCGCC2346800.017.13716550-54
GTGGTCG2403300.017.02752750-54
CCGTGTA2698800.015.28033835-39
GAGCCGT2769650.014.68003835-39
Produced by FastQC (version 0.11.5)