FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409687138
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATG41214651.0060030246787977TruSeq Adapter, Index 1 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA8267750.081.775721
TCGGAAG8320750.081.410052
CGGAAGA8550000.079.193993
AGAGCAC10314950.065.6295247
AGCACAC10421000.064.812119
GAGCACA10602500.063.8434338
GAAGAGC11120750.061.041055
AAGAGCA13344250.050.9931836
GGAAGAG14308050.047.701854
TATGCCG5756750.023.77406745-49
CTCGTAT5657300.023.16102840-44
CGTATGC5850250.023.0783745-49
TCTCGTA5716950.022.90237840-44
ATGCCGT6021250.022.76150945-49
GCCGTCT6009550.022.55003250-54
TGCCGTC6000550.022.49402645-49
TCGTATG5832600.022.14028440-44
CGTCTTC6192400.022.1330650-54
CCGTCTT6227900.021.98384950-54
ATCTCGT5965300.021.92313440-44
Produced by FastQC (version 0.11.5)