FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445145527
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1212650.032.3364379
GAGCGTC1588700.024.9240238
ATCGGAA1754100.022.8690091
TCGGAAG1900200.021.1968172
AGAGCGT1919950.020.7484057
CGGAAGA2167100.018.6757723
AAGAGCG2330800.017.2900356
CGCCGTA729850.011.21849755-59
TCGCCGT874500.09.49568955-59
GCCGTAT974300.08.62138255-59
GAAGAGC5158100.08.4056635
GTCGCCG995100.08.38718555-59
TGGTCGC1136150.07.489112450-54
CCGTATC1109900.07.451647855-59
CGTATCA1172600.07.32746560-64
GCGTCGT1142500.07.11183910-14
GGTCGCC1172950.07.08243250-54
GTGGTCG1240800.06.962579750-54
CGTCGTG1196350.06.855920310-14
CCGTGTA1460750.05.980702435-39
Produced by FastQC (version 0.11.5)