FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452331934
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCTC10375020.2293674007990778Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2078600.078.55699
GAGCGTC2489400.065.771398
ATCGGAA2593700.064.14631
TCGGAAG2773300.059.8491862
AGAGCGT2795750.058.645137
CGGAAGA3005700.055.1615683
AAGAGCG3220900.051.2754976
GAAGAGC5990100.028.1249755
CGCCGTA1537300.021.32033255-59
TCGCCGT1721700.019.07895555-59
GCCGTAT1791900.018.45691955-59
GGAAGAG9578800.017.9633624
GTCGCCG1851400.017.84339555-59
CCGTATC1918500.016.91099455-59
TGGTCGC2001900.016.67538650-54
CGTATCA1981500.016.61682760-64
GGTCGCC2036150.016.10508550-54
GCGTCGT2078400.016.07421910-14
CGTCGTG2130400.015.70228710-14
GTGGTCG2143600.015.6177250-54
Produced by FastQC (version 0.11.5)