FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347500524
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG9142760.2631006104612377TruSeq Adapter, Index 13 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2778800.057.2211571
TCGGAAG2836550.056.0088122
CGGAAGA2959450.053.7433053
AGAGCAC4826750.033.314357
AGCACAC4995250.032.1601149
GAGCACA5112850.031.495558
GAAGAGC5317600.030.3335655
AAGAGCA7366600.022.1364886
TATGCCG1517300.021.37160345-49
GGAAGAG8150650.020.1605454
CGTATGC1616900.019.84080945-49
CTCGTAT1497750.019.29415940-44
TCTCGTA1520800.018.9969640-44
ATGCCGT1717150.018.865745-49
TGCCGTC1684900.018.79309545-49
GCCGTCT1713500.018.60810350-54
TCGTATG1612400.017.5742540-44
ATCTCGT1699500.017.09074240-44
CGTCTTC1929200.016.78309650-54
CCGTCTT1946750.016.65190750-54
Produced by FastQC (version 0.11.5)