FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423086253
Sequences flagged as poor quality0
Sequence length151
%GC40

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC93010652.1983850654679626Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG11653850.0125.344099
GAGCGTC11987800.0121.787148
ATCGGAA12314950.0118.990451
AGAGCGT12350650.0117.9374857
TCGGAAG12484200.0117.308862
CGGAAGA12694600.0115.29523
AAGAGCG12762500.0114.22216
GAAGAGC15403050.094.818385
GGAAGAG18884650.077.713484
CGCCGTA10425650.027.93459155-59
TCGCCGT10534900.027.55159255-59
GCCGTAT10678750.027.3071355-59
GTCGCCG10677200.027.09477655-59
CCGTATC10776150.026.85903455-59
TGGTCGC10875050.026.85672650-54
GGTCGCC10836050.026.73730750-54
CGTATCA10868850.026.60802760-64
GTGGTCG11117450.026.29697650-54
TCTCGGT11359000.025.90431245-49
CTCGGTG11508450.025.54070545-49
Produced by FastQC (version 0.11.5)