FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004953808

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953808
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435835722
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG6861740.15743867823665908TruSeq Adapter, Index 7 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1770100.067.0644451
TCGGAAG1913250.061.9860732
CGGAAGA2123650.055.8311543
AGAGCAC4616200.026.1005367
AGCACAC4904600.024.5804639
GAGCACA5011900.024.1381028
GAAGAGC5133950.023.5940045
TATGCCG1256900.019.72543145-49
CGTATGC1378500.017.84130545-49
TCGTATC1393200.017.81146435-39
CTCGTAT1288300.017.73744840-44
TCTCGTA1334400.017.07576640-44
GTCACGA1506000.016.555525-29
AAGAGCA7503350.016.4043436
TATCTCG1383150.016.31561940-44
ATGCCGT1549550.016.00754745-49
ATTCGTA1604450.015.45818635-39
GCCGTCT1598500.015.2503650-54
TGCCGTC1568500.015.12356545-49
TCGTATG1453450.015.04759640-44
Produced by FastQC (version 0.11.5)